Complete microbe free processed porcine xenograft for clinical use
Complete microbe free processed porcine xenograft for clinical use
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DOI：
10.1007/s12055-007-0049-y
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发表时间：
2007-12-01
影响因子：
0.7
通讯作者：
Guhathakurta, Soma
中科院分区：
文献类型：
--
作者：
Balasundari, Ramesh;Gupta, Ruchi;Guhathakurta, Soma
背景：在保证无菌性和免疫原性的前提下，从猪到人的异种器官移植是可能的。本研究的目的是检查加工过的猪肺异种移植物的无菌性，以标记其“安全”用于临床应用，而不会对异种人畜共患病造成威胁。方法：对148根猪肺瓣膜导管进行处理前后细菌、真菌和病毒污染筛查。从预处理的猪管道中分离出念珠菌、丝状真菌、酵母样生物、革兰氏阴性菌和猪内源性逆转录病毒（PERV）等细菌和真菌污染物。这些收获的导管被收集在抗菌剂和汉克斯平衡盐溶液的混合物中。接下来，它们进行“加工”，即使其在所有方面都完全无细胞，坚固且具有生物相容性。最后，进行细菌、真菌和病毒（PERV）污染的无菌检查。结果：本研究的微生物评价表明，经特殊的分阶段脱细胞技术处理后，猪肺导管是“无微生物”的。结论：实验室制备的猪异种移植物导管具有数量和尺寸上的优势，可安全替代金标准同种移植物。
Background: Xenogenic organ replacement from pig to human is a possibility provided sterility and immunogenicity are taken care of. The objective of this study was to check the sterility of processed porcine pulmonary xenografts to mark them 'safe' for clinical application, without the threat for xenozoonoses.Methods: A total of 148 porcine pulmonary valve conduits were screened pre and post processing for bacterial, fungal and viral contamination. The bacterial and fungal contaminants such as Candida species, filamentous fungus, yeast like organisms, gram negative bacteria and porcine endogenous retrovirus (PERV) were isolated from the preprocessed porcine conduits. These harvested conduits were collected in a cocktail of antimicrobials and Hanks balanced salt solution. Next, they were subjected to 'processing' i. e., made completely acellular, strong and biocompatible in all respects. Finally, sterility checks were carried out for bacterial, fungal and viral (PERV) contamination.Result: Microbial evaluation performed in this study revealed that the porcine pulmonary conduits were made 'microbe free' on subjecting to the special staged decellularization technique.Conclusion: The porcine xenograft conduit processed in the laboratory, which has an added advantage of numbers and sizes, can replace the gold standard homograft safely.