Antimicrobial Susceptibility and Clonality of Clinical Ureaplasma Isolates in the United States
Antimicrobial Susceptibility and Clonality of Clinical Ureaplasma Isolates in the United States
复制标题
DOI：
10.1128/aac.00671-16
复制
发表时间：
2016-08-01
影响因子：
4.9
通讯作者：
Patel, Robin
中科院分区：
文献类型：
--
作者：
Fernandez, Javier;Karau, Melissa J.;Patel, Robin
解脲支原体和微小解脲支原体是泌尿生殖道和宫内感染以及新生儿和免疫抑制患者全身性疾病的病原体。关于这些物种的抗菌敏感性和克隆性的信息有限。在这项研究中，我们报告了在明尼苏达州罗切斯特市梅奥诊所回收的 250 种当代解脲脲原体分离株（202 株微小脲原体分离株和 48 株解脲支原体分离株）的敏感性。多西环素、阿奇霉素、环丙沙星、四环素、红霉素和左氧氟沙星的 MIC 通过肉汤微量稀释法测定，最后三种的 MICS 根据 CLSI 指南进行解释。分别有 6.4% 和 5.2% 的小 U. parvum 和 5.2% 的 U. urealyticum 分离株发现了左氧氟沙星耐药性，而分别有 27.2% 和 68.8% 的分离株显示环丙沙星 MIC ≥ 4 μ g/ml。左氧氟沙星耐药菌株的耐药机制是由于parC突变所致，其中Ser83Leu取代最常见，其次是Glu87Lys。在研究的250个分离株中未发现大环内酯类耐药性；单个小 U. parvum 分离株具有四环素抗性。在 10 个微小 U. parvum 分离株中发现了 tet(M)，其中包括单一的四环素抗性分离株，以及 9 个四环素和多西环素 MIC 较低的分离株。对 46 个分离株进行的多位点序列分型 (MLST) 显示出所研究的临床解脲支原体分离株的高度多样性，无论抗菌药物敏感性如何。目前的工作扩展了先前关于美国解脲支原体物种对抗菌药物的敏感性、耐药机制和克隆性的知识。
Ureaplasma urealyticum and Ureaplasma parvum are pathogens involved in urogenital tract and intrauterine infections and also in systemic diseases in newborns and immunosuppressed patients. There is limited information on the antimicrobial susceptibility and clonality of these species. In this study, we report the susceptibility of 250 contemporary isolates of Ureaplasma (202 U. parvum and 48 U. urealyticum isolates) recovered at Mayo Clinic, Rochester, MN. MICs of doxycycline, azithromycin, ciprofloxacin, tetracycline, erythromycin, and levofloxacin were determined by broth microdilution, with MICS of the last three interpreted according to CLSI guidelines. Levofloxacin resistance was found in 6.4% and 5.2% of U. parvum and U. urealyticum isolates, respectively, while 27.2% and 68.8% of isolates, respectively, showed ciprofloxacin MICs of >= 4 mu g/ml. The resistance mechanism of levofloxacin-resistant isolates was due to mutations in parC, with the Ser83Leu substitution being most frequent, followed by Glu87Lys. No macrolide resistance was found among the 250 isolates studied; a single U. parvum isolate was tetracycline resistant. tet(M) was found in 10 U. parvum isolates, including the single tetracycline-resistant isolate, as well as in 9 isolates which had low tetracycline and doxycycline MICs. Multilocus sequence typing (MLST) performed on a selection of 46 isolates showed high diversity within the clinical Ureaplasma isolates studied, regardless of antimicrobial susceptibility. The present work extends previous knowledge regarding susceptibility to antimicrobial agents, resistance mechanisms, and clonality of Ureaplasma species in the United States.