Paternal epigenetic effects of population density on locust phase-related characteristics associated with heat-shock protein expression
Paternal epigenetic effects of population density on locust phase-related characteristics associated with heat-shock protein expression
复制标题
种群密度对与热休克蛋白表达相关的蝗虫阶段相关特征的父系表观遗传效应
DOI：
10.1111/mec.13072
复制
发表时间：
2015-02-01
影响因子：
4.9
通讯作者：
Kang, Le
中科院分区：
文献类型：
--
作者：
Chen, Bing;Li, Shaoqin;Kang, Le
许多物种表现出跨代可塑性，亲本中的任何一方所经历的环境线索都可以传递给后代，从而导致后代的表型变异与祖先的环境相匹配。然而，在动物中，父系经历通过表观遗传影响后代可塑性的方式通常仍不清楚。在本研究中，我们研究了种群密度对迁徙蝗虫阶段相关性状的跨代影响。通过明确控制遗传背景的实验设计，我们发现群体或隔离饲养对相可塑性的影响可以遗传给后代。群居蝗虫的隔离导致后代卵的重量减少和孵化物形态特征的改变，而孤立蝗虫的拥挤则表现出相反的效果。密度变化的结果通过母系和父系遗传传递，尽管父系效应的表达不像母系效应那样明显。热休克蛋白（Hsps）的显著表达，如Hsp90、Hsp70和Hsp20.6，可由密度变化触发。热休克蛋白在拥挤条件下显著上调，在隔离条件下显著下调。亲本Hsp表达的变异也遗传给子代，遗传模式与期相特征一致。这些结果揭示了种群密度对期多表型和热蛋白表达的影响，并为研究环境变化的父本表观遗传提供了一个模型系统。
Many species exhibit transgenerational plasticity by which environmental cues experienced by either parent can be transmitted to their offspring, resulting in phenotypic variants in offspring to match ancestral environments. However, the manner by which paternal experiences affect offspring plasticity through epigenetic inheritance in animals generally remains unclear. In this study, we examined the transgenerational effects of population density on phase-related traits in the migratory locust Locusta migratoria. Using an experimental design that explicitly controls genetic background, we found that the effects of crowd or isolation rearing on phase plasticity could be inherited to the offspring. The isolation of gregarious locusts resulted in reduced weight in offspring eggs and altered morphometric traits in hatchlings, whereas crowding of solitarious locusts exhibited opposite effects. The consequences of density changes were transmitted by both maternal and paternal inheritance, although the expression of paternal effects was not as pronounced as that of maternal effects. Prominent expression of heat-shock proteins (Hsps), such as Hsp90,Hsp70 and Hsp20.6, could be triggered by density changes. Hsps were significantly upregulated upon crowding but downregulated upon isolation. The variation in parental Hsp expression was also transmitted to the offspring, in which the pattern of inheritance was consistent with that of phase characteristics. These results revealed a paternal effect on phase polyphenism and Hsp expression induced by population density, and defined a model system that could be used to study the paternal epigenetic inheritance of environmental changes.