Expression of N-Acetyl Galactosaminylated and Sialylated Glycans by Metastases Arising from Primary Breast Cancer
Expression of N-Acetyl Galactosaminylated and Sialylated Glycans by Metastases Arising from Primary Breast Cancer
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原发性乳腺癌转移灶中 N-乙酰半乳糖胺化和唾液酸化聚糖的表达
DOI：
10.1159/000024504
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Leathem
中科院分区：
文献类型：
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作者：
S. Brooks;A. Leathem
本研究探讨了Hessopomatia凝集素（HPA）的结合特性所产生的原发性乳腺癌的转移，并比较HPA的结合模式与结合的双花扁豆凝集素（DBA），Limax黄凝集素（LFA），并对Tn表位的单克隆抗体。在46个个体的尸检中，在一系列组织中的81个转移块中，79%为HPA阳性。未观察到HPA结合的位点特异性。HPA阳性和阴性肿瘤沉积物均见于单个个体。HPA阳性肿瘤通常对唾液酸特异性凝集素LFA的结合呈阴性（86%为阴性）。α-GalNAc特异性HPA和DBA与抗Tn表位单克隆抗体（GalNAc-O-Ser/Thr）的结合模式明显不同。
This study examines the Helix pomatia lectin (HPA) binding characteristics of metastases arising from primary breast cancer, and compares HPA binding patterns with binding of Dolichos biflorus lectin (DBA), Limax flavus lectin (LFA), and a monoclonal antibody against the Tn epitope. Of 81 blocks of metastases in a range of tissues, taken at autopsy from 46 individuals, 79% were HPA positive. No site specificity with regard to HPA binding was observed. Both HPA-positive and -negative tumour deposits were seen within a single individual. HPA-positive tumours were commonly negative for binding of sialic acid specific lectin LFA (86% were negative). Binding patterns of α-GalNAc specific HPA and DBA, and a monoclonal antibody against Tn epitope (GalNAc-O-Ser/Thr) were markedly different.