The Vibrio cholerae cytolysin promotes activation of mast cell (T helper 2) cytokine production
The Vibrio cholerae cytolysin promotes activation of mast cell (T helper 2) cytokine production
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DOI：
10.1111/j.1462-5822.2007.01092.x
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发表时间：
2008-04-01
影响因子：
3.4
通讯作者：
de Bernard, Marina
中科院分区：
文献类型：
--
作者：
Arcidiacono, Diletta;Odom, Sandra;de Bernard, Marina
许多霍乱弧菌菌株产生溶细胞素（VCC），其形成寡聚跨膜孔，负责培养中几种细胞类型的空泡化。在这里，我们认为，VCC可能有助于在自然感染中看到的辅助性T细胞2（Th 2）反应;通过TLR 2，VCC增强肥大细胞分泌IL-4，IL-6和TNF-α分别为330-，290-和550-倍。此外，VCC诱导的细胞因子产生依赖于胞质Ca 2+的增加和Src家族激酶林恩和Fyn的存在，已知这是肥大细胞的Fc β RI依赖性活化所需的。这些发现强烈表明，VCC具有促炎活性，促进Th 2型免疫特征。
Many strains of Vibrio cholerae produce a cytolysin (VCC) that forms oligomeric transmembrane pores responsible for vacuolization of several cell types in culture. Here we suggest that VCC could contribute to the T helper 2 (Th2) response seen in the natural infection; acting through TLR2, VCC enhances mast cells secretion of IL-4, IL-6 and TNF-alpha by 330-, 290- and 550-fold respectively. Moreover, VCC-induced cytokine production is dependent on increased cytosolic Ca2+ and on the presence of the Src family kinases Lyn and Fyn, known to be required for Fc epsilon RI-dependent activation of mast cells. These findings strongly suggest that VCC has a pro-inflammatory activity promoting a Th2-type immune profile.