ELECTRON-MICROSCOPY OF U1 SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES - SHAPE OF THE PARTICLE AND POSITION OF THE 5' RNA TERMINUS
ELECTRON-MICROSCOPY OF U1 SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES - SHAPE OF THE PARTICLE AND POSITION OF THE 5' RNA TERMINUS
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DOI：
10.1002/j.1460-2075.1989.tb03374.x
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发表时间：
1989-01-01
期刊：
影响因子：
11.4
通讯作者：
LUHRMANN, R
中科院分区：
文献类型：
--
作者：
KASTNER, B;LUHRMANN, R
用双碳膜负染法在电镜下观察了HeLa细胞核内核糖核蛋白小颗粒U_1（U_1snRNP）。对U1 snRNP颗粒的数百个视图的调查显示，直径约为8 nm的主体具有两个长4-7 nm和宽3-4 nm的特征突起。然后使用抗m3 G抗体来确定U1 snRNA的5“帽中m3 G碱基的位点，该位点靠近与前体mRNA的5”剪接序列发生碱基配对的位点。m3 G碱基位于U1 snRNP的主体上，靠近其中一个突起，大致与另一个突起相对。
Small nuclear ribonucleoprotein particles U1 (U1 snRNPs) from HeLa cells were purified to homogeneity and examined in the electron microscope by negative staining with the double carbon film technique. A survey of several hundred views of the U1 snRNP particles revealed a main body about 8 nm in diameter with two characteristic protuberances 4-7 nm long and 3-4 nm wide. Anti-m3G antibodies were then used to define the site of the m3G base in the 5'' cap of the U1 snRNA, close to the site where base-pairing occurs with the 5'' splice sequence of pre-mRNA. The m3G base is located on the body of the U1 snRNP, close to one of the protuberances and approximately opposite the other.