Vascular density and phenotype around ductal carcinoma in situ (DCIS) of the breast.
Vascular density and phenotype around ductal carcinoma in situ (DCIS) of the breast.
复制标题
乳房原位（DCIS）周围的导管癌周围的血管密度和表型。
DOI：
10.1038/sj.bjc.6600053
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发表时间：
2002-03-18
影响因子：
8.8
通讯作者：
Holcombe, C
中科院分区：
文献类型：
--
作者：
Teo, N B;Shoker, B S;Jarvis, C;Martin, L;Sloane, J P;Holcombe, C
高达50%的乳腺导管原位癌复发与浸润性癌有关，但尚未发现病理或分子特征可预测浸润性疾病的发展。肿瘤要想侵袭，需要形成新的血管。以前的研究已经描述了导管周围的血管环与导管原位癌有关，这增加了导管周围血管形成的特征在确定从原位疾病向侵袭性疾病转化方面的重要作用的可能性。用形态计量学和一组抗内皮细胞抗体(von Willebrand因子、CD31、CD141和CD34)测定导管周围血管密度和表型，并与浸润性癌的存在和其他组织学特征相关。与正常小叶相比，单纯导管原位癌中CD34+和CD31+血管密度较高，而vWF免疫阳性血管密度较低，说明两者在表型和密度上存在差异。导管原位癌合并浸润性癌的血管免疫染色特征与单纯导管原位癌相似，但CD34+和CD141+血管数明显增多，vWF染色较少。血管密度与受累导管横截面积及肿瘤坏死程度呈显著负相关。血管密度和核级别之间的相关性也被注意到，在中等级别中最高。与浸润性癌相关的导管原位癌周围CD34+和CD141+血管密度较高，可反映较大的侵袭倾向，但不能完全排除与浸润性癌共存的直接影响。血管密度、分级、导管大小和核分级之间的关系表明，导管周围血管生成随着肿瘤生长速度的增加而增加，但无法跟上最快速增长的病变的步伐。《英国癌症杂志》(2002)86,905-911。DOI：10.1038/sj/bjc/6600053 www.bjancer.com2002英国癌症研究中心
Up to 50% of recurrences of ductal carcinoma in situ of the breast are associated with invasive carcinoma but no pathological or molecular features have yet been found to predict for the development of invasive disease. For a tumour to invade, it requires the formation of new blood vessels. Previous studies have described a vascular rim around ducts involved by ductal carcinoma in situ, raising the possibility that the characteristics of periductal vascularisation may be important in determining transformation from in situ to invasive disease. Periductal vascular density and phenotype were determined using morphometry and a panel of anti-endothelial antibodies (von Willebrand factor, CD31, CD141 and CD34) and related to the presence of invasive carcinoma and other histological features. Compared to normal lobules, pure ductal carcinoma in situ exhibited a greater density of CD34+ and CD31+ vessels but a decrease in those that were immunopositive for vWF, indicating a difference in phenotype and in density. Ductal carcinoma in situ associated with invasive carcinoma showed a profile of vascular immunostaining similar to that of pure ductal carcinoma in situ but there were significantly greater numbers of CD34+ and CD141+ vessels and fewer staining for vWF. There was a significant negative correlation between vascular density and both the cross-sectional areas of the ducts involved and the extent of the necrosis of the tumour they contained. A correlation between vascular density and nuclear grade was also noted, being highest in the intermediate grade. The greater density of CD34+ and CD141+ vessels around ductal carcinoma in situ associated with invasive carcinoma could reflect a greater predisposition to invade but a direct effect of co-existent invasive carcinoma cannot entirely be ruled out in the present study. The relationship between vascular density, grade, duct size and nuclear grade suggests that periductal angiogenesis increases with tumour growth rate but is unable to keep pace with the most rapidly growing lesions. British Journal of Cancer (2002) 86, 905–911. DOI: 10.1038/sj/bjc/6600053 www.bjcancer.com © 2002 Cancer Research UK