Triplex staples: DNA double-strand cross-linking at internal and terminal sites using psoralen-containing triplex-forming oligonucleotides
Triplex staples: DNA double-strand cross-linking at internal and terminal sites using psoralen-containing triplex-forming oligonucleotides
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DOI：
10.1021/bc0601875
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发表时间：
2006-11-15
影响因子：
4.7
通讯作者：
Brown, Tom
中科院分区：
文献类型：
--
作者：
Li, Hong;Broughton-Head, Victoria J.;Brown, Tom
已开发了一种在固相寡核苷酸合成期间将4 '-（羟甲基）-4，5'，8-三甲基噻吩连接至胸腺嘧啶碱基的5位的方法。紫外线照射含有内部连接的peptien的三链体形成寡核苷酸（TFO）在靶双链体内的TpA步骤处产生光加合物，从而放松对peptien靶序列选择的限制。含有位于5 '和3'末端的两个多聚体的TFO的光反应已用于在TFO的两个末端产生双链交联（三链体斯台普斯）。这种复合物没有游离的单链末端。含有4 '-（羟甲基）-4，5'，8-三甲基戊烯、3-甲基-2-氨基吡啶和5-（3-氨基丙-2-炔基）脱氧尿苷的TFO在接近生理条件下与靶双链体形成光加合物。
A method has been developed to attach 4'-( hydroxymethyl)- 4,5', 8- trimethylpsoralen to the 5 position of thymine bases during solid- phase oligonucleotide synthesis. UV irradiation of triplex- forming oligonucleotides ( TFOs) containing internally attached psoralens produces photoadducts at TpA steps within target duplexes, thus relaxing the constraints on selection of psoralen target sequences. Photoreaction of TFOs containing two psoralens, located at the 5'- and 3'- ends, has been used to create double-strand cross- links ( triplex staples) at both termini of the TFO. Such complexes have no free single-stranded ends. TFOs containing 4'-( hydroxymethyl)- 4,5', 8- trimethylpsoralen, 3- methyl- 2- aminopyridine, and 5-( 3- aminoprop- 2- ynyl) deoxyuridine formed photoadducts with target duplexes under near- physiological conditions.