Prominin-1 Localizes to the Open Rims of Outer Segment Lamellae in Xenopus laevis Rod and Cone Photoreceptors
Prominin-1 Localizes to the Open Rims of Outer Segment Lamellae in Xenopus laevis Rod and Cone Photoreceptors
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DOI：
10.1167/iovs.11-8635
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发表时间：
2012-01-01
影响因子：
4.4
通讯作者：
Papermaster, David S.
中科院分区：
文献类型：
--
作者：
Han, Zhou;Anderson, David W.;Papermaster, David S.
目的. Prominin-1在视杆细胞和视锥细胞中均有表达。视网膜变性是视网膜色素变性的一个重要原因。在这项研究中，作者研究了xlProminin-1蛋白的表达和亚细胞定位，xlProminin-1蛋白是非洲爪蟾（Xenopus laevis）的同源蛋白，在这种蛙的视杆和视锥光感受器中。产生对xlProminin-1特异的抗体。采用免疫印迹技术研究xlProminin-1蛋白的表达和翻译后加工。用免疫细胞化学光镜、电镜及转基因技术研究xlProminin-1在细胞内的分布。x1 Prominin-1在视网膜、脑和肾中表达并进行翻译后蛋白水解加工。xlProminin-1在X染色体的视杆细胞和视锥细胞的外节中表达和定位不同。光滑。特异性针对xlProminin-1的N或C末端的抗体标记了视锥外节（COS）的层的开放边缘和视杆外节（ROS）基部的层的开放边缘。与此相反，抗外周蛋白-2/rds抗体Xper 5A 11标记了与纤毛轴丝相邻的锥板的闭合边缘和闭合ROS盘的边缘。抗xlProminin-1抗体标记基础ROS的程度随该蛙的光周期而变化。整个ROS也被两种抗体微弱地标记，这一结果与目前的观点形成对比，即Ros-1仅定位于基底ROS。这些结果表明，xlProminin-1可能作为一个抗融合因子在盘形态发生的调节，并可能有助于维持开放的层状结构的基础ROS和COS盘在X。光感受器（Invest Ophthalmol维斯科学。2012; 53：361-373）DOI：10.1167/iovs.11-8635
PURPOSE. Prominin-1 expresses in rod and cone photoreceptors. Mutations in the prominin-1 gene cause retinal degeneration in humans. In this study, the authors investigated the expression and subcellular localization of xlProminin-1 protein, the Xenopus laevis ortholog of prominin-1, in rod and cone photoreceptors of this frog.METHODS. Antibodies specific for xlProminin-1 were generated. Immunoblotting was used to study the expression and posttranslational processing of xlProminin-1 protein. Immunocytochemical light and electron microscopy and transgenesis were used to study the subcellular distribution of xlProminin-1.RESULTS. xlProminin-1 is expressed and is subject to posttranslational proteolytic processing in the retina, brain, and kidney. xlProminin-1 is differently expressed and localized in outer segments of rod and cone photoreceptors of X. laevis. Antibodies specific for the N or C termini of xlProminin-1 labeled the open rims of lamellae of cone outer segments (COS) and the open lamellae at the base of rod outer segments (ROS). By contrast, anti-peripherin-2/rds antibody, Xper5A11, labeled the closed rims of cone lamellae adjacent to the ciliary axoneme and the rims of the closed ROS disks. The extent of labeling of the basal ROS by anti-xlProminin-1 antibodies varied with the light cycle in this frog. The entire ROS was also faintly labeled by both antibodies, a result that contrasts with the current notion that prominin-1 localizes only to the basal ROS.CONCLUSIONS. These findings suggest that xlProminin-1 may serve as an anti-fusogenic factor in the regulation of disk morphogenesis and may help to maintain the open lamellar structure of basal ROS and COS disks in X. laevis photoreceptors. (Invest Ophthalmol Vis Sci. 2012; 53: 361-373) DOI:10.1167/iovs.11-8635