Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection
Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection
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DOI：
10.1016/j.jfms.2007.04.003
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发表时间：
2007-12-01
影响因子：
1.7
通讯作者：
Egberink, Herman F.
中科院分区：
文献类型：
--
作者：
Hartmann, Katrin;Griessmayr, Pascale;Egberink, Herman F.
许多用于鉴定猫免疫缺陷病毒（FIV）和猫白血病病毒（FeLV）感染的新的诊断内部测试已获准用于兽医实践，这些试剂盒的相对优点的问题促使了比较研究。本研究的目的是确定七个FIV和八个FeLV测试的优势和劣势，这是商业上可用的。在这项研究中，随机选择536只猫的血清样本进行了测试。在至少一项试验中呈fiv阳性的样品经Western blot证实，felv阳性的样品经病毒分离证实。此外，随机选择在所有检测系统中检测为阴性的样本，通过Western blot（100个样本）和病毒分离（81个样本）重新检测。比较各试验的特异性、敏感性、阳性预测值和阴性预测值及结果质量。
Many new diagnostic in-house tests for identification of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infection have been licensed for use in veterinary practice, and the question of the relative merits of these kits has prompted comparative studies. This study was designed to define the strengths and weaknesses of seven FIV and eight FeLV tests that are commercially available. In this study, 536 serum samples from randomly selected cats were tested. Those samples reacting FIV-positive in at least one of the tests were confirmed by Western blot, and those reacting FeLV-positive were confirmed by virus isolation. In addition, a random selection of samples testing negative in all test systems was re-tested by Western blot (100 samples) and by virus isolation (81 samples). Specificity, sensitivity, positive and negative predictive values of each test and the quality of the results were compared.