Developing a tissue engineered repair material for treatment of stress urinary incontinence and pelvic organ prolapsewhich cell source?
Developing a tissue engineered repair material for treatment of stress urinary incontinence and pelvic organ prolapsewhich cell source?
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DOI：
10.1002/nau.22443
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发表时间：
2014-06-01
影响因子：
2
通讯作者：
MacNeil, Sheila
中科院分区：
文献类型：
--
作者：
Roman, Sabiniano;Mangera, Altaf;MacNeil, Sheila
目的 合成不可吸收网片广泛用于增强压力性尿失禁（SUI）和盆腔器官脱垂（POP）的手术修复；然而，人们越来越担心这种网格会导致严重的并发症。本研究比较了两种自体细胞来源在可生物降解支架上附着和细胞外基质 (ECM) 生产的潜力，以开发组织工程修复材料 (TERM)。方法分离人口腔成纤维细胞（OF）和人脂肪干细胞（ADSC），并在热退火聚左旋乳酸（PLA）支架上在无约束或约束（有或没有间歇性应激）条件下培养两周。测试样品的细胞代谢活性（AlamarBlue (R) 测定）、收缩（使用 image J 软件分析系列照片）、总胶原蛋白生成（天狼星红测定）和 ECM 成分的生成（胶原蛋白 I、III 和弹性蛋白的免疫染色；以及扫描电子显微镜）和生物力学特性（BOSE 张力计）。使用两个样本 t 检验对差异进行统计检验。结果两种细胞在支架上均表现出良好的附着和增殖。在相同条件下，具有 ADSC 的无约束支架比 OF 产生更多的总胶原蛋白和更致密的均质 ECM。限制条件（有或没有间歇性压力）产生相似的总胶原蛋白产量，但提高了两种细胞的弹性蛋白产量，尤其是 OF。与未接种的支架相比，在支架上添加任何细胞都会导致支架的生物力学性能增加。结论 OF 和 ADSC 似乎都是适合与可生物降解支架结合的细胞类型，用于开发治疗 SUI 和 POP 的 TERM。神经尿素。尿动力学。 33:531-537，2014 年。(c) 2013 年 Wiley 期刊公司。
Aims Synthetic non-absorbable meshes are widely used to augment surgical repair of stress urinary incontinence (SUI) and pelvic organ prolapse (POP); however, there is growing concern such meshes are associated with serious complications. This study compares the potential of two autologous cell sources for attachment and extra-cellular matrix (ECM) production on a biodegradable scaffold to develop tissue engineered repair material (TERM). Methods Human oral fibroblasts (OF) and human adipose-derived stem cells (ADSC) were isolated and cultured on thermo-annealed poly-L-lactic acid (PLA) scaffolds for two weeks under either unrestrained conditions or restrained (either with or without intermittent stress) conditions. Samples were tested for cell metabolic activity (AlamarBlue (R) assay), contraction (serial photographs analyzed with image J software), total collagen production (Sirius red assay), and production of ECM components (immunostaining for collagen I, III, and elastin; and scanning electron microscopy) and biomechanical properties (BOSE tensiometer). Differences were statistically tested using two sample t-test. Results Both cells showed good attachment and proliferation on scaffolds. Unrestrained scaffolds with ADSC produced more total collagen and a denser homogenous ECM than OF under same conditions. Restrained conditions (both with and without intermittent stress) gave similar total collagen production, but improved elastin production for both cells, particularly OF. The addition of any cell onto scaffolds led to an increase in biomechanical properties of scaffolds compared to unseeded scaffolds. Conclusions OF and ADSC both appear to be suitable cell types to combine with biodegradable scaffolds, in the development of a TERM for the treatment of SUI and POP. Neurourol. Urodynam. 33:531-537, 2014. (c) 2013 Wiley Periodicals, Inc.