Superparamagnetic iron oxide labeling of stem cells for MRI tracking and delivery in cardiovascular disease.
Superparamagnetic iron oxide labeling of stem cells for MRI tracking and delivery in cardiovascular disease.
复制标题
干细胞的超顺磁铁标记，用于心血管疾病中的MRI跟踪和递送。
DOI：
10.1007/978-1-60761-705-1_11
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Kraitchman, Dara L
中科院分区：
文献类型：
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作者：
Kedziorek, Dorota A;Kraitchman, Dara L
在20世纪80年代中期，氧化铁纳米颗粒被开发为诊断成像的造影剂。在过去的二十年里，已经建立了用超顺磁性氧化铁(SPIO)标记细胞的方法，以帮助定向递送和追踪干细胞疗法。在心血管应用的细胞治疗临床试验激增的情况下，为了了解心血管再生治疗和干细胞的生物分布机制，SPIO标记的干细胞的临床前动物研究的数量也出现了类似的上升。采用有限数量的SPIO直接标记干细胞的方法，在很大程度上是因为希望迅速将这些技术转化为临床试验。在这篇综述中，我们将概述最常用的心血管干细胞氧化铁标记方法，并描述心脏中磁性标记细胞的磁共振成像(MRI)策略。
In the mid-1980s, iron oxide nanoparticles were developed as contrast agents for diagnostic imaging. In the last two decades, established methods to label cells with superparamagnetic iron oxides (SPIOs) have been developed to aid in targeted delivery and tracking of stem cell therapies. The surge in cellular therapy clinical trials for cardiovascular applications has seen a similar rise in the number of preclinical animal studies of SPIO-labeled stem cells in an effort to understand the mechanisms of cardiovascular regenerative therapy and stem cell biodistribution. The adoption of a limited number of methods of direct labeling of stem cells with SPIOs is due in large part to the desire to rapidly translate these techniques to clinical trials. In this review, we will outline the most commonly adopted methods for iron oxide labeling of stem cells for cardiovascular applications and describe strategies for magnetic resonance imaging (MRI) of magnetically labeled cells in the heart.