DNA FINGERPRINTING OF TINOSPORA CORDIFOLIA USING RAPD ANALYSIS
DNA FINGERPRINTING OF TINOSPORA CORDIFOLIA USING RAPD ANALYSIS
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使用 RAPD 分析对青牛胆进行 DNA 指纹分析
DOI：
10.1234/jgpt.v2i2.122
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
K. Dhalwal
中科院分区：
文献类型：
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作者：
Vaibhav Mukund Shinde;K. Dhalwal
青牛胆是印度传统医学系统阿育吠陀中重要的免疫调节剂之一。本研究利用随机扩增多态性DNA（RAPD）技术对青牛胆不同样品进行遗传多态性分析。分析良好表征的标记化合物是现在最流行的方法，用于鉴定和质量控制的草药材料。然而，在许多草药物种中，植物的化学成分随着环境生长和加工条件而变化，这降低了鉴别方法的可靠性。为了确定遗传多态性，使用120个十聚体寡核苷酸引物筛选20个选定的样品。用乙醇沉淀法从茎中提取总DNA，作为PCR反应的模板。4个引物OPA-16、OPC-7、OPC-13和OPG-5均表现出明显的多态性。该引物对采集的20份青牛胆样品具有较好的检测能力。它可用于检测制剂中药物的存在。RAPD分析结果表明，青牛胆样品间遗传多样性较低。
Tinospora cordifolia Linn. is one of the important immunomodulating agents in Ayurveda, the traditional Indian system of medicine. In this study, we analyze different samples of Tinospora cordifolia for genetic polymorphism using Random Amplified Polymorphic DNA (RAPD) markers. Analysis of well-characterized marker compounds is now the most popular method for identifying and quality control of herbal materials. However, in many herbal species the chemical composition of the plant changes with environment growing and processing conditions, which lowers the reliability of authentification methods. For the determination of genetic polymorphism, one hundred and twenty decamer oligonucleotide primers were used to screen twenty selected samples. Total DNA extracted from the stem by using ethanol precipitation was used as the template in the PCR reaction. Four primers OPA-16, OPC-7, OPC-13 and OPG-5, showed reproducible distinct polymorphic patterns. The primers possess abilities in detecting variations in twenty collected samples of Tinospora cordiofolia. It can be applied to detect the presence of the drug in formulations. A low genetic diversity among the Tinospora cordifolia samples was revealed by RAPD analysis.