Long-term ventilatory adaptation and ventilatory response to hypoxia in plateau pika (Ochotona curzoniae): role of nNOS and dopamine
Long-term ventilatory adaptation and ventilatory response to hypoxia in plateau pika (Ochotona curzoniae): role of nNOS and dopamine
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DOI：
10.1152/ajpregu.00108.2009
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发表时间：
2009-10-01
影响因子：
2.8
通讯作者：
Ge, Ri-Li
中科院分区：
文献类型：
--
作者：
Pichon, Aurelien;Bai Zhenzhong;Ge, Ri-Li
[10] Pichon A，Zhenzhong B，Favret F，Jin G，Shufeng H，Marchant D，Richalet JP，Ge RL.高原鼠兔（Ochotona curzoniae）的长期通气适应和对缺氧的通气反应：nNOS和多巴胺的作用。Am J Physiol Regul Integr Comp Physiol 297：R978-R987，2009。首次发表于2009年7月29日; doi：10.1152/ajpregu.00108.2009。我们评估了重复暴露于低氧洞穴的高海拔（HA）高原鼠兔和对照大鼠的呼吸模式和对低氧的呼吸反应（HVR）。我们分别用S-甲基-L-硫代瓜氨酸（SMTC）抑制剂和氟哌啶醇拮抗剂评价神经元型一氧化氮合酶（nNOS）和多巴胺的作用。使用全身体积描记器测量清醒鼠兔（n = 9）和低海拔（LA）大鼠（n = 7）在不同PI（O2）（56、80、111、150和186 mmHg）下的通气量（（V）/dotI），以及HA适应大鼠（n = 9，在4，600 m处8天）在两种不同PIO（2）（56和80 mmHg）下的通气量。在PI（O2）= 56和80 mmHg时，评估了NaCl、SMTC和氟哌啶醇对鼠兔呼吸模式的影响。我们观察到主要的种属效应，鼠兔的dotI、潮气量（VT）、吸气时间/总时间（T（i）/T（tot））较大，呼气时间较LA大鼠短。高原鼠兔在缺氧状态下的VT较HA大鼠大，呼吸频率较HA大鼠低。鼠兔和大鼠的HVR无统计学差异。在鼠兔中，当PI（O2）为56 mmHg时，SMTC诱导（V）显著增加，超过dotI和VT，但当PIO（2）为80 mmHg时无影响，即，鼠兔的生存高度在鼠兔中，氟哌啶醇注射对任何排泄参数均无影响。鼠兔的长期适应性适应主要是由于呼吸模式（VT和T（i）/T（tot））的改善，而HVR没有显著改善。鼠兔对严重急性缺氧的敏感性似乎受到外周nNOS机制的调节。
Pichon A, Zhenzhong B, Favret F, Jin G, Shufeng H, Marchant D, Richalet JP, Ge RL. Long-term ventilatory adaptation and ventilatory response to hypoxia in plateau pika (Ochotona curzoniae): role of nNOS and dopamine. Am J Physiol Regul Integr Comp Physiol 297: R978-R987, 2009. First published July 29, 2009; doi: 10.1152/ajpregu.00108.2009.-We assessed ventilatory patterns and ventilatory responses to hypoxia (HVR) in high-altitude (HA) plateau pikas, repetitively exposed to hypoxic burrows, and control rats. We evaluated the role of neuronal nitric oxide synthase (nNOS) and dopamine by using S-methyl-L-thiocitrulline (SMTC) inhibitor and haloperidol antagonist, respectively. Ventilation ((V) over dotI) was measured using a whole body plethysmograph in conscious pikas (n = 9) and low-altitude (LA) rats (n = 7) at different PI(O2) (56, 80, 111, 150, and 186 mmHg) and in HA acclimatized rats (n = 9, 8 days at 4,600 m) at two different PIO(2) (56 and 80 mmHg). The effects of NaCl, SMTC, and haloperidol on ventilatory patterns were assessed in pikas at PI(O2) = 56 and 80 mmHg. We observed a main species effect with larger (V) over dotI, tidal volume (VT), inspiratory time/total time (T(i)/T(tot)), and a lower expiratory time in pikas than in LA rats. Pikas had also a larger VT and lower respiratory frequency compared with HA rats in hypoxia. HVR of pikas and rats were not statistically different. In pikas, SMTC induced a significant increase in (V) over dotI and VT for a PI(O2) of 56 mmHg, but had no effect for a PIO(2) of 80 mmHg, i.e., the living altitude of pikas. In pikas, haloperidol injection had no effect on any ventilatory parameter. Long-term ventilatory adaptation in pikas is mainly due to an improvement in respiratory pattern (VT and T(i)/T(tot)) with no significant improvement in HVR. The sensitivity to severe acute hypoxia in pikas seems to be regulated by a peripheral nNOS mechanism.