Functional human endogenous retroviral LTR transcription start sites are located between the R and U5 regions
Functional human endogenous retroviral LTR transcription start sites are located between the R and U5 regions
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DOI：
10.1016/j.virol.2005.11.007
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发表时间：
2006-03-15
期刊：
影响因子：
3.7
通讯作者：
Sverdlov, E
中科院分区：
文献类型：
--
作者：
Kovalskaya, E;Buzdin, A;Sverdlov, E
人类内源性逆转录病毒 (HERV) 约占人类 DNA 的 5%，被认为是人类祖先基因细胞的古代逆转录病毒感染的残余物。 HERV 可以通过集中在长末端重复序列 (LTR) 中的顺式调控元件来改变宿主细胞基因的表达。尽管在人类转录组中鉴定出了许多与 HERV 相关的 RNA，但对于其中的大多数，仍不清楚它们是 LTR 启动子还是从邻近基因组启动子启动的通读产物。在这里，我们描述了内源性逆转录病毒 HERV-K (HML-2) 人类特异性亚科的孤立和前病毒 LTR 内转录起始位点的图谱。令人惊讶的是..转录主要从 LTR R 区域的 3' 末端起始。这里提供的数据可能有助于揭示人内源性逆转录病毒与其宿主细胞的适应性协同进化。 (C) 2005 Elsevier Inc. 保留所有权利。
Human endogenous retroviruses (HERVs) occupy about 5% of human DNA and are thought to be remnants of ancient retroviral infections of human ancestors' genii cells. HERVs can modify expression of host cell genes through their cis-regulatory elements concentrated in their long terminal repeats (LTRs). Although numerous HERV-related RNAs were identified in the human transcriptome, for most of them, it remains unclear whether they are LTR-promoted or read-through products initiated from neighboring genomic promoters. Here, we describe mapping of transcriptional start sites within solitary and proviral LTRs of the HERV-K (HML-2) human-specific subfamily of endogenous retroviruses. Surprisingly.. the transcription was initiated predominantly from the very 3' termini of the LTR R regions. The data presented here may shed light on adaptive coevolution of human endogenous retroviruses with their host cells. (C) 2005 Elsevier Inc. All rights reserved.