Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials
Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials
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DOI：
10.1016/s0142-9612(97)00109-9
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发表时间：
1998-03-01
期刊：
影响因子：
14
通讯作者：
Truskey, GA
中科院分区：
文献类型：
--
作者：
Burmeister, JS;Olivier, LA;Truskey, GA
细胞粘附和功能取决于细胞和基质之间粘附接触的形成。细胞-基质接触面积的测定是必要的，以了解生物材料的性质如何影响细胞粘附。在这篇综述中，我们描述了全内反射荧光显微镜（TIRFM）的发展和应用，以量化细胞与生物材料表面的分离距离。提出了一种近似理论，用于当荧光剂被放置在细胞膜中时直接计算分离距离。这种方法的有效性进行了讨论。TIRFM相比，干涉反射显微镜和相关技术，测量细胞/基板分离距离。然后，这种方法被应用到一些重要的问题，在细胞基质的相互作用，包括在接触面积和粘附强度的变化，生物材料表面上，结合强度的分析，和实时测量细胞/基板分离距离后，暴露于流。(C)1998爱思唯尔科技有限公司版权所有。
Cell adhesion and function depend upon the formation of adhesive contacts between the cell and substrate. Determination of the cell-substrate contact area is necessary in order to understand how biomaterial properties influence cell adhesion. In this review we describe the development and application of total internal reflection fluorescence microscopy (TIRFM) to quantify the separation distance of cells from a biomaterial surface. An approximate theory is presented for the straightforward calculation of separation distances when a fluor is placed in the cell membrane. The validity of this approach is discussed. TIRFM is compared to interference reflection microscopy and related techniques that measure cell/substrate separation distances. This approach is then applied to a number of important problems in cell substrate interactions, including changes in contact area and adhesion strength on biomaterial surfaces, analysis of bond strength, and real-time measurement of cell/substrate separation distances following exposure to flow. (C) 1998 Elsevier Science Ltd. All rights reserved.