Relationship between the microbiota in different sections of the gastrointestinal tract, and the body weight of broiler chickens.
Relationship between the microbiota in different sections of the gastrointestinal tract, and the body weight of broiler chickens.
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DOI：
10.1186/s40064-016-2604-8
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kong C
中科院分区：
文献类型：
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作者：
Han GG;Kim EB;Lee J;Lee JY;Jin G;Park J;Huh CS;Kwon IK;Kil DY;Choi YJ;Kong C
在家禽业中，为进一步提高肉鸡的生长性能已经进行了许多努力，鉴定和调节与体重（BW）相关的细菌可能是提高生产力的策略之一。然而，关于微生物群与体重之间关系的研究很少。本研究的目的是探讨胃肠道不同部位微生物群与体重的关系。根据体重选择18日龄候鸟20只，分别从胃肠道的嗉囊、回肠和盲肠3个不同部位采集样本。提取细菌基因组DNA，扩增16S rRNA基因V4区。扩增子在Illumina MiSeq上测序，并用QIIME分析微生物群落。在主坐标分析中，基于GIT的部分，细菌群落聚类为三组。通过线性回归分析确定了几个与bw相关的细菌群。在属水平上，回肠链球菌以及回肠和盲肠中的Akkermansia与体重呈负相关，而回肠中的双歧杆菌和盲肠中的乳球菌与体重呈正相关。本研究结果表明，胃肠道中特定的细菌群落与体重有关，拓宽了对肉鸡肠道微生物生态系统的认识。本文的在线版本（doi:10.1186/s40064-016-2604-8）包含补充材料，仅供授权用户使用。
In the poultry industry, many efforts have been undertaken to further improve the growth performance of broilers and identification and modulation of body weight (BW)-related bacteria could be one of the strategies to improve productivity. However, studies regarding the relationship between microbiota and BW are scarce. The objective of the present study was to investigate the relationship between microbiota and BW in different sections of the gastrointestinal tract (GIT). A total of twenty 18-day-old birds were selected based on the BW, and samples were collected from the three different sections of the GIT, which included the crop, ileum and cecum. Bacterial genomic DNA was extracted from the samples, and the V4 region of 16S rRNA gene were amplified. Amplicons were sequenced on Illumina MiSeq, and microbial communities were analyzed by using QIIME. In principal coordinate analysis, bacterial communities were clustered into three groups, based on the sections of GIT. Several BW-related bacterial groups were identified from linear regression analysis. At the genus level, Streptococcus from the ileum as well as Akkermansia in both ileum and cecum, were negatively related to BW, whereas Bifidobacterium in the ileum and Lactococcus in the cecum showed a positive correlation. The results from the present study showed that particular bacterial communities in the GIT were related to BW, and the study has broadened the understanding of the intestinal microbial ecosystem in broiler chickens. The online version of this article (doi:10.1186/s40064-016-2604-8) contains supplementary material, which is available to authorized users.