Rapid method for chromatin immunoprecipitation (ChIP) assay in a dimorphic fungus, Candida albicans
Rapid method for chromatin immunoprecipitation (ChIP) assay in a dimorphic fungus, Candida albicans
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DOI：
10.1007/s12275-020-9143-2
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发表时间：
2020-01-01
影响因子：
3
通讯作者：
Lee, Jung-Shin
中科院分区：
文献类型：
--
作者：
Kim, Jueun;Lee, Jung-Shin
染色质免疫沉淀（ChIP）试验是一种鉴定目标蛋白与DNA区域结合程度的方法。这种方法对于研究转录因子或染色质修饰如何调控基因表达的机制是必不可少的。白色念珠菌是一种二型致病真菌，它可以响应环境信号迅速地从酵母菌转变为菌丝。C.的形态变化白念珠菌是其毒力的关键因素之一。因此，了解C.白色念珠菌改变其形态。我们了解这种调节的基本方法之一是ChIP测定。已经进行了许多努力来优化方案以降低背景信号并准确地分析结果，因为ChIP测定可以提供非常不同的结果，即使在实验程序中存在轻微差异。我们已经优化了快速和有效的ChIP协议，使它可以同样适用于酵母和菌丝形式的C。白色念珠菌。我们的方法在这个协议中也是比较快速的方法广泛使用。在本方案中，我们描述了我们的快速方法，在C。白色的细节
A chromatin immunoprecipitation (ChIP) assay is a method to identify how much a protein of interest binds to the DNA region. This method is indispensable to study the mechanisms of how the transcription factors or chromatin modifications regulate the gene expression. Candida albicans is a dimorphic pathogenic fungus, which can change its morphology very rapidly from yeast to hypha in response to the environmental signal. The morphological change of C. albicans is one of the critical factors for its virulence. Therefore, it is necessary to understand how to regulate the expression of genes for C. albicans to change its morphology. One of the essential methods for us to understand this regulation is a ChIP assay. There have been many efforts to optimize the protocol to lower the background signal and to analyze the results accurately because a ChIP assay can provide very different results even with slight differences in the experimental procedure. We have optimized the rapid and efficient ChIP protocol so that it could be applied equally for both yeast and hyphal forms of C. albicans. Our method in this protocol is also comparatively rapid to the method widely used. In this protocol, we described our rapid method for the ChIP assay in C. albicans in detail.