Osteoprogenitor response to defined topographies with nanoscale depths
Osteoprogenitor response to defined topographies with nanoscale depths
复制标题
DOI：
10.1016/j.biomaterials.2005.08.028
复制
发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Oreffo, ROC
中科院分区：
文献类型：
--
作者：
Dalby, MJ;McCloy, D;Oreffo, ROC
在用于承重应用的下一代骨科植入物的开发中，影响骨祖细胞群体活动和功能的能力将是非常可取的。这将允许硬组织直接形成在植入物上，即骨整合，而不是形成纤维囊膜，后者形成在许多当前的非生物活性假体周围。由于模数失配，包膜的形成会导致微动，最终导致骨折，需要进行翻修手术。微地形和最近的纳米拓扑术已被证明对多种类型的细胞的黏附、增殖和基因表达产生影响。这项研究考察了使用一系列纳米级的浅坑和沟槽来调节细胞黏附的可能性。纳米拓扑图是用光刻技术制造的，然后生产镍垫片，最后压印成聚甲基丙烯酸甲酯。对人类骨祖细胞进行的细胞测试表明，纳米拓扑图可以控制细胞黏附、细胞骨架、生长和成骨细胞标志物骨钙素和骨桥蛋白的产生。结论：人骨髓基质细胞对纳米尺度的特征具有高度的响应性。(C)2005爱思唯尔有限公司。保留所有权利。
In the development of the next generation of orthopaedic implants for load-bearing applications, an ability to influence osteoprogenitor population activity and function will be highly desirable. This will allow the formation of hard-tissue directly onto the implant, i.e. osseointegration, rather than the formation of fibrous capsules which form around many of the current, non-bioactive, prosthesis. The formation of capsules leads to micromotion due to modulus mismatch and ultimately to fracture and the need for revision surgery.Microtopography and latterly nanotopography have been shown to elicit influence over adhesion, proliferation and gene expression of a wide number of cell types. This study has examined the possibility of modulating cell adhesion using a range of nanometric scale shallow pits and grooves. The topographies were manufactured using photolithography followed by the production of nickel shims and finally embossing into polymethylmethacrylate.Cell testing with human osteoprogenitor populations showed that the nanotopographies allowed control of cell adhesion, cytoskeleton, growth and production of the osteoblastic markers osteocalcin and osteopontin. It is concluded that the human bone marrow stromal cells are highly responsive to nanoscale features. (c) 2005 Elsevier Ltd. All rights reserved.