Quantitative estimates of outcrossing rates in a natural population of Caldesia grandis (Alismataceae)
Quantitative estimates of outcrossing rates in a natural population of Caldesia grandis (Alismataceae)
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DOI：
10.1007/s11859-006-0004-6
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发表时间：
2007-03
影响因子：
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通讯作者：
Jinming Chen;W. R. Gituru;Qingfeng Wang
中科院分区：
文献类型：
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作者：
Jinming Chen;W. R. Gituru;Qingfeng Wang
使用 24 个个体的 10 个开放授粉后代阵列，通过显性随机扩增多态性 DNA (RAPD) 标记估计了大白花自然群体的异交率。基于所有 25 个 RAPD 位点估计的多位点异交率为 0.872 ± 0.033，单位点异交率为 0.795 ± 0.032。多位点估计与单位点估计没有显着差异。根据 RAPD 数据估计的后代中的固定指数 F 为 -0.142 ±0.000。从 MLDT 获得的多位点异交率 (tm) 和单位点异交率 (ts) 的估计值清楚地表明，异交在开放授粉 C 中占主导地位。庞大的人口。实证分析表明，15 个应该是实现稳健估计所需的最小显性标记位点数量。
Outcrossing rate in a natural population ofCaldesia grandiswas estimated by the dominant random amplified polymorphism DNA (RAPD) marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ± 0.033 and the single-locus outcrossing rate was 0.795 ± 0.032. Multilocus estimates did not differ significantly from the single-locus estimates. The fixation index,F, in the progeny estimated from RAPD data was −0.142 ±0.000. The estimates of multilocus outcrossing rates (tm) and single-locus outcrossing rates (ts) obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinatedC. grandispopulation. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates oftm.