Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA.
Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA.
复制标题
spen蛋白FPA导致拟南芥NLR基因普遍提前转录终止。
DOI：
10.7554/elife.65537
复制
发表时间：
2021-04-27
期刊：
影响因子：
7.7
通讯作者：
Simpson GG
中科院分区：
文献类型：
--
作者：
Parker MT;Knop K;Zacharaki V;Sherwood AV;Tomé D;Yu X;Martin PG;Beynon J;Michaels SD;Barton GJ;Simpson GG
参与抗病的基因是基因组中进化最快、最多样化的组成部分。在植物基因组中发现了大量核苷酸结合的富亮氨酸重复序列（NLR）基因，这些基因是抗病性所必需的。然而，NLRs会引发自身免疫，破坏有益微生物群或降低适应性。因此，了解nlr是如何控制的是至关重要的。在这里，我们发现rna结合蛋白FPA介导NLR转录本广泛的过早裂解和聚腺苷化，从而控制其功能表达并影响免疫。利用长读纳米孔直接RNA测序，我们解决了NLR转录加工和基因注释的复杂性。我们的研究结果揭示了NLR控制的共转录层，这对理解NLR在植物免疫应答中的调节和进化动力学具有重要意义。
Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here, we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.