H+-GRADIENT-DEPENDENT ACTIVE-TRANSPORT OF TETRAETHYLAMMONIUM CATION IN APICAL-MEMBRANE VESICLES ISOLATED FROM KIDNEY EPITHELIAL-CELL LINE LLC-PK1
H+-GRADIENT-DEPENDENT ACTIVE-TRANSPORT OF TETRAETHYLAMMONIUM CATION IN APICAL-MEMBRANE VESICLES ISOLATED FROM KIDNEY EPITHELIAL-CELL LINE LLC-PK1
复制标题
DOI：
10.1042/bj2270199
复制
发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
HORI, R
中科院分区：
文献类型：
--
作者：
INUI, K;SAITO, H;HORI, R
用培养的肾上皮细胞系LLC-PK1的顶膜小泡研究了有机阳离子[~3H]四乙基铵(Net~(4+))的转运。顶膜囊泡对NET4+的吸收具有渗透敏感性、时间依赖性和可饱和性。H+梯度([H+]1>；[H+]0)对其浓度梯度有明显的刺激作用(超调现象)，而HgCl2抑制了这种集中吸收。从LLC-PK1细胞分离的顶膜保留了Net4+的运输特性，类似于在肾刷状缘膜中观察到的。
Transport of [3H]tetraethylammonium (NEt4+), an organic cation, was studied by using apical-membrane vesicles isolated from cultured kidney epithelial cell line LLC-PK1. The uptake of NEt4+ by apical-membrane vesicles was osmotically sensitive, time-dependent and saturable. The presence of an H+ gradient ([H+]1 > [H+]0) induced a marked stimulation of NEt4+ uptake against its concentration gradient (overshoot phenomenon), and this concentrative uptake was inhibited by HgCl2. Apical membranes isolated from the LLC-PK1 cells retain the transport characteristics of NEt4+ similar to those observed in renal brush-border membranes.