UV-B-Induced CPD Photolyase Gene Expression is Regulated by UVR8-Dependent and -Independent Pathways in Arabidopsis.
UV-B-Induced CPD Photolyase Gene Expression is Regulated by UVR8-Dependent and -Independent Pathways in Arabidopsis.
复制标题
DOI：
10.1093/pcp/pcv121
复制
发表时间：
2015-10
影响因子：
4.9
通讯作者：
Nan Li;Mika Teranishi;Hiroko Yamaguchi;Tomonao Matsushita;M. Watahiki;T. Tsuge;Shao-shan Li;J. H
中科院分区：
文献类型：
--
作者：
Nan Li;Mika Teranishi;Hiroko Yamaguchi;Tomonao Matsushita;M. Watahiki;T. Tsuge;Shao-shan Li;J. H
植物已经进化出各种机制来保护免受UV-B辐射（280-315 nm）对生长和发育的有害影响。环丁烷嘧啶二聚体（CPD）光解酶是UV-B诱导CPD的修复酶，对保护细胞免受UV-B辐射至关重要。CPD光裂合酶基因（PHR）的表达受包括UV-B在内的各种波长的光的控制，但这种调节的机制仍然知之甚少。在这项研究中，我们研究了不同波长的光，特别是低通量UV-B辐射（280 nm，0.2 µmol m（-2）s（-1）），在光暗循环下生长7 d，然后适应黑暗3 d的拟南芥幼苗中PHR表达的调控。低通量UV-B辐射诱导CPD，而不是活性氧。UV-B、UV-A（375 nm）和蓝光均能有效诱导AtPHR的表达。由UV-A和蓝光诱导的表达主要由隐花色素依赖的途径调节，而光敏色素A和B起着次要但明显的作用。UV-B诱导的表达主要由UVR 8依赖性途径调节。AtPHR的表达也介导的UVR 8的非依赖性途径，这是相关的CPD积累UV-B辐射诱导。这些结果表明，拟南芥已经进化出不同的机制来调节CPD光裂合酶的表达，通过多种光感受器信号通路，包括UVR 8依赖性和非依赖性途径，作为对UV-B辐射的有害影响的保护。
Plants have evolved various mechanisms that protect against the harmful effects of UV-B radiation (280-315 nm) on growth and development. Cyclobutane pyrimidine dimer (CPD) photolyase, the repair enzyme for UV-B-induced CPDs, is essential for protecting cells from UV-B radiation. Expression of the CPD photolyase gene (PHR) is controlled by light with various wavelengths including UV-B, but the mechanisms of this regulation remain poorly understood. In this study, we investigated the regulation of PHR expression by light with various wavelengths, in particular low-fluence UV-B radiation (280 nm, 0.2 µmol m(-2) s(-1)), in Arabidopsis thaliana seedlings grown under light-dark cycles for 7 d and then adapted to the dark for 3 d. Low-fluence UV-B radiation induced CPDs but not reactive oxygen species. AtPHR expression was effectively induced by UV-B, UV-A (375 nm) and blue light. Expression induced by UV-A and blue light was predominantly regulated by the cryptochrome-dependent pathway, whereas phytochromes A and B played a minor but noticeable role. Expression induced by UV-B was predominantly regulated by the UVR8-dependent pathway. AtPHR expression was also mediated by a UVR8-independent pathway, which is correlated with CPD accumulation induced by UV-B radiation. These results indicate that Arabidopsis has evolved diverse mechanisms to regulate CPD photolyase expression by multiple photoreceptor signaling pathways, including UVR8-dependent and -independent pathways, as protection against harmful effects of UV-B radiation.