Isolation and molecular characterization of equine H3N8 influenza viruses from pigs in China
Isolation and molecular characterization of equine H3N8 influenza viruses from pigs in China
复制标题
中国猪源马 H3N8 流感病毒的分离和分子鉴定
DOI：
10.1007/s00705-009-0381-1
复制
发表时间：
2009-05-01
影响因子：
2.7
通讯作者：
Jin, Meilin
中科院分区：
文献类型：
--
作者：
Tu, Jiagang;Zhou, Hongbo;Jin, Meilin
在2004-2006年猪流感病毒监测期间，从中国中部的猪中分离出两株H3N8流感病毒。对8个基因片段的序列和系统发育分析表明，这2株猪分离株起源于马，与20世纪90年代初的欧洲马H3N8流感病毒最密切相关。比较血凝素（HA）氨基酸序列发现了几个重要的取代。一个取代导致潜在糖基化位点的丢失，两个取代，分别位于切割位点和受体结合袋附近，之前在犬H3 ha中已经报道过。具有HA蛋白突变的马H3N8流感病毒宿主范围的扩大可能会提高这些病毒向人类传播的可能性。
During 2004-2006 swine influenza virus surveillance, two strains of H3N8 influenza viruses were isolated from pigs in central China. Sequence and phylogenetic analyses of eight gene segments revealed that the two swine isolates were of equine origin and most closely related to European equine H3N8 influenza viruses from the early 1990s. Comparison of hemagglutinin (HA) amino acid sequences showed several important substitutions. One substitution caused the loss of a potential glycosylation site, and two substitutions, located at the cleavage site and adjacent to the receptor-binding pocket, respectively, had been reported previously in canine H3 HAs. This expansion of host range of equine H3N8 influenza viruses with mutations in the HA protein might raise the possibility of transmission of these viruses to humans.