Hyaluronan, heterogeneity, and healing: The effects of ultrapure hyaluronan of defined molecular size on the repair of full-thickness pig skin wounds
Hyaluronan, heterogeneity, and healing: The effects of ultrapure hyaluronan of defined molecular size on the repair of full-thickness pig skin wounds
复制标题
DOI：
10.1046/j.1524-475x.1995.30310.x
复制
发表时间：
1995-01-01
影响因子：
2.9
通讯作者：
West, David C.
中科院分区：
文献类型：
--
作者：
Arnold, Frank;Jia, Chiyu;West, David C.
细胞外基质大分子透明质酸被认为调节伤口愈合。然而，透明质酸的分子大小和污染相关蛋白可能是这些作用的重要决定因素。我们已经检查了七个每日局部治疗的全层皮肤伤口的猪与超纯透明质酸定义的分子大小的结果。高分子量透明质酸（gt 1000 kd）与中等分子量透明质酸（分子量= 100 kd）和生理盐水对照相比，增加了早期伤口收缩率，而低分子量透明质酸则降低了早期伤口收缩率。21天的断裂强度被高分子量和中等分子量透明质酸降低，但不被低分子量透明质酸降低。通过扫描激光多普勒技术作为血管生成的非侵入性指标测量的伤口灌注在第3天显示高分子量和中等分子量透明质酸的抑制，但所有形式的透明质酸在第7天引起血流量升高。在该湿愈合模型中，肉芽组织的结构高度组织化，但未观察到由于处理而导致的大体组织学差异。不同分子种类的透明质酸对收缩、血管生成和伤口强度的演变具有不同的作用。如果透明质酸被用作伤口的治疗剂或载体，则应详细说明其确切成分。
The extracellular matrix macromolecule, hyaluronan, is thought to modulate wound healing. However, the molecular size of hyaluronan and contaminating associated proteins may be important determinants of these effects. We have examined the results of seven daily topical treatments of full-thickness skin wounds in pigs with ultrapure hyaluronan of defined molecular size. High molecular weight hyaluronan ( gt 1000 kd) enhanced, whereas low molecular weight hyaluronan decreased, the rate of early wound contraction as compared with intermediate hyaluronan (molecular weight = 100 kd) and saline solution controls, Fracture strength at 21 days was reduced by high and intermediate molecular weight hyaluronan but not by low molecular weight hyaluronan. Wound perfusion, measured by means of a scanning laser-Doppler technique as a noninvasive indicator of angiogenesis, showed depression by high and intermediate molecular weight hyaluronan on day 3, but all forms of hyaluronan caused elevated blood flow on day 7. The architecture of granulation tissue in this wet healing model-was highly organized, but no gross histologic differences were seen because of treatment. Different molecular species of hyaluronan have differential effects on contraction, angiogenesis, and the evolution of wound strength. Where hyaluronan is used as a treatment or vehicle for wounds, its precise composition should be specified.