Isolation and flow cytometric analysis of T‐cell‐depleted CD34+ PBPCs
Isolation and flow cytometric analysis of T‐cell‐depleted CD34+ PBPCs
复制标题
T 细胞耗尽的 CD34+ PBPC 的分离和流式细胞术分析
作者：
J. Debelak;M. Shlomchik;E. Snyder;D. Cooper;S. Seropian;Joel McGuirk;B. Smith;D. Krause
背景：为了将同种异体HPC移植扩展到更大范围的患者，部分匹配的相关供体的使用正在开发中。由于此类移植的组织相容性固有地较高，因此GVHD和移植失败的风险增加。从PBPC中体外清除供体来源的T淋巴细胞是预防GVHD的最有效方法之一。到目前为止，个别中心已经使用定制开发的程序来消耗负责同种异体反应性的T细胞移植物，通常使用相对不纯的非标准化试剂，如大豆凝集素和补体。此外，随着T细胞耗竭方法的改进，很难准确评估剩余的T细胞数量。由于不同的中心使用不同的方案来检测T细胞，因此很难在机构之间重现和验证结果，这限制了中心之间数据的直接比较。
BACKGROUND: To extend allogeneic HPC transplantation to a greater range of patients, the use of partially matched related donors is under development. Because of the inherently higher degree of histoincom‐patibility in such transplants, there is increased risk of GVHD as well as of graft failure. Ex vivo depletion of donor‐derived T‐lymphocytes from PBPCs is one of the most effective methods of preventing GVHD. Thus far, individual centers have used custom‐developed procedures to deplete the graft of T cells that are responsible for alloreactivity, often employing relatively impure, nonstandardized reagents such as soybean agglutinin and complement. In addition, with improved methods of T‐cell depletion, it has been difficult to accurately assess the number of T cells remaining. Because different centers have used different protocols to assay T cells, it has been difficult to reproduce and validate the results between institutions, and this has limited direct comparison of data between centers.
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影响因子：
20.3
作者：
R. Champlin;W. Ho;J. Gajewski;S. Feig;M. Burnison;G. Holley;P. Greenberg;Kyoung Lee;I. Schmid;J. Giorgi
通讯作者：
R. Champlin;W. Ho;J. Gajewski;S. Feig;M. Burnison;G. Holley;P. Greenberg;Kyoung Lee;I. Schmid;J. Giorgi
慢性粒细胞白血病骨髓移植的适应症。
影响因子：
20.3
作者：
Thomas,ED;Clift,RA
通讯作者：
Clift,RA
用抗 CD5 单克隆抗体选择性清除骨髓 T 淋巴细胞：有效预防血液系统恶性肿瘤患者的移植物抗宿主病。
影响因子：
20.3
作者：
Antin,JH;Bierer,BE;Smith,BR;Ferrara,J;Guinan,EC;Sieff,C;Golan,DE;Macklis,RM;Tarbell,NJ;Lynch,E
通讯作者：
Lynch,E
再生障碍性贫血骨髓移植的变化趋势。
影响因子：
4.8
作者：
Storb,R;Longton,G;Anasetti,C;Appelbaum,FR;Beatty,P;Bensinger,W;Crawford,S;Deeg,HJ;Doney,K;Fefer,A
通讯作者：
Fefer,A
同种异体骨髓移植治疗慢性粒细胞白血病。
DOI：
10.1200/jco.1987.5.7.1033
发表时间：
1987
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
McGlave,P;Arthur,D;Haake,R;Hurd,D;Miller,W;Vercellotti,G;Weisdorf,D;Kim,T;Ramsay,N;Kersey,J
通讯作者：
Kersey,J