Genetic dissection of harvest index and related traits through genome-wide quantitative trait locus mapping in Brassica napus L.
Genetic dissection of harvest index and related traits through genome-wide quantitative trait locus mapping in Brassica napus L.
复制标题
通过甘蓝型油菜全基因组数量性状基因座作图对收获指数和相关性状进行遗传解析。
DOI：
10.1270/jsbbs.18115
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发表时间：
2019-01-01
期刊：
影响因子：
2.4
通讯作者：
Li, Maoteng
中科院分区：
文献类型：
--
作者：
Chao, Hongbo;Raboanatahiry, Nadia;Li, Maoteng
收获指数（HI）是粮食产量与总生物量的比值，代表农作物的可收获产量。甘蓝型油菜的HI低于其他重要经济作物，对此问题进行的相关研究有限。本研究通过对11个相关性状的表型分析，揭示了HI的复杂性以及培育高HI优良品种的可行性。基于高密度遗传图谱的数量性状基因座（QTL）定位，确定了 HI 的 160 个 QTL、163 个上位位点对以及种子产量（SY）、生物量产量（BY）和株高（PH）三个密切相关的性状，其中 HI、SY 和 PH 分别包括 2 个、5 个和 3 个主要 QTL。鉴定并研究了具有编码区变异的QTL和上位位点下的相关候选基因，包括与OsTB1同源的BnaA02g14010D，其作为侧枝负调节因子，以及与控制粒宽和重量的OsGW2同源的BnaA02g18890D。 HI 与相关性状、大量 QTL 和上位位点以及此处确定的候选基因的复杂相关性为 HI 的遗传结构提供了新的见解，这可能进一步增强油菜产量提高的有效育种策略。
The harvest index (HI) is the ratio of grain yield to the total biomass and represents the harvestable yield of crops. In Brassica napus, the HI is lower than that of other economically important crops, and limited relevant studies have been carried out regarding this issue. In this study, phenotypic analyses of 11 related traits showed the complexity of HI and the feasibility of cultivating desirable varieties with high HI. Quantitative trait loci (QTL) mapping based on a high-density genetic map identified 160 QTL, 163 epistatic loci pairs for HI and three closely related traits: seed yield (SY), biomass yield (BY) and plant height (PH), including two, five and three major QTL for HI, SY and PH, respectively. The related candidate genes underlying the QTL and epistatic loci with coding region variation were identified and investigated, including BnaA02g14010D, homologous to OsTB1, which functions as a negative regulator for lateral branching, and BnaA02g18890D, homologous to OsGW2, which controls grain width and weight. The complex correlation of HI with related traits, numerous QTL and epistatic loci and the candidate genes identified here provide new insights into the genetic architecture of HI, which might further enhance effective breeding strategies for yield improvement in rapeseed.