Automatic classification of activity and apneas using whole body plethysmography in newborn mice
Automatic classification of activity and apneas using whole body plethysmography in newborn mice
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DOI：
10.1152/japplphysiol.00803.2004
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发表时间：
2005-01-01
影响因子：
3.3
通讯作者：
Gallego, J
中科院分区：
文献类型：
--
作者：
Matrot, B;Durand, E;Gallego, J
越来越多的新生小鼠研究正在进行，以确定睡眠呼吸暂停的机制，这是早期呼吸障碍的标志。全身体积描记法是首选的方法，因为它不需要固定，这会影响行为状态和呼吸。然而，体积描记器内部的活动可能干扰呼吸信号。将呼吸信号视觉分类为呼吸活动、活动相关紊乱或呼吸暂停是如此耗时，以至于显著妨碍了大型幼犬样品的表型分型。我们提出了一个自动分类的活动的基础上呼吸紊乱和呼吸暂停的频谱分析的基础上。该方法在新生小鼠出生当天和出生后第2、5和10天，在常氧和缺氧（5%O-2）条件下进行了验证。对于活动和呼吸暂停，视觉和自动评分显示出高Pearson相关系数（分别为0.92和0.98）和高组内相关系数（0.96 - 0.99），支持两种方法之间的高度一致性。目前的研究结果表明，呼吸紊乱可以提供一个有效的活动在自由移动的新生小鼠的间接指数，自动呼吸暂停分类的基础上频谱分析可能是有效的精度和节省的时间。
An increasing number of studies in newborn mice are being performed to determine the mechanisms of sleep apnea, which is the hallmark of early breathing disorders. Whole body plethysmography is the method of choice, as it does not require immobilization, which affects behavioral states and breathing. However, activity inside the plethysmograph may disturb the respiratory signal. Visual classification of the respiratory signal into ventilatory activity, activity- related disturbances, or apneas is so time-consuming as to considerably hamper the phenotyping of large pup samples. We propose an automatic classification of activity based on respiratory disturbances and of apneas based on spectral analysis. This method was validated in newborn mice on the day of birth and on postnatal days 2, 5, and 10, under normoxic and hypoxic (5% O-2) conditions. For both activity and apneas, visual and automatic scores showed high Pearson's correlation coefficients (0.92 and 0.98, respectively) and high intraclass correlation coefficients (0.96 - 0.99), supporting strong agreement between the two methods. The present results suggest that breathing disturbances may provide a valid indirect index of activity in freely moving newborn mice and that automatic apnea classification based on spectral analysis may be efficient in terms of precision and of time saved.