Effect of PBAN on pheromone production by mated Heliothis virescens and Heliothis subflexa females
Effect of PBAN on pheromone production by mated Heliothis virescens and Heliothis subflexa females
复制标题
DOI：
10.1007/s10886-005-0970-8
复制
发表时间：
2005-01-01
影响因子：
2.3
通讯作者：
Schal, C
中科院分区：
文献类型：
--
作者：
Groot, AT;Fan, YL;Schal, C
通过注射信息素生物合成激活神经肽（PBAN），诱导雌花和花花在光期产生性信息素。当注射1 pmol Hez-PBAN时，在光期从交配雌虫腺体中提取的信息素总量与在光期从未交配雌虫腺体中提取的信息素总量相似。在春季和秋季，比较了雄性雌性、注射pban的雌性和处女雌性的信息素成分谱。在春季和秋季，处女雌虫的信息素混合物的相对组成存在一定的差异，但在注射了pban的交配雌虫中没有发现这种时间差异。由于处女雌虫信息素混合成分的时间变化比注射了PBAN的雌虫更大，因此PBAN可以用来确定雌性的天然信息素表型。这种方法的优点是，信息素腺体可以在光期提取，从已经产卵的雌性交配。
Mated female Heliothis virescens and H. subflexa were induced to produce sex pheromone during the photophase by injection of pheromone biosynthesis activating neuropeptide (PBAN). When injected with 1 pmol Hez-PBAN, the total amount of pheromone that could be extracted from glands of mated females during the photophase was similar to that extracted from virgin females in the scotophase. The PBAN-induced profile of pheromone components was compared between mated, PBAN-injected females and virgin females during spring and fall. Virgin females exhibited some differences in the relative composition of the pheromone blend between spring and fall, but no such temporal differences were detected in PBAN-injected, mated females. Because the temporal variation in pheromone blend composition was greater for virgin females than for PBAN-injected females, PBAN can be used to determine a female's native pheromone phenotype. This procedure has the advantages that pheromone glands can be extracted during the photophase, from mated females that have already oviposited.