1H NMR spectroscopy of rat brain in vivo at 14.1 Tesla: Improvements in quantification of the neurochemical profile
1H NMR spectroscopy of rat brain in vivo at 14.1 Tesla: Improvements in quantification of the neurochemical profile
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DOI：
10.1016/j.jmr.2008.06.019
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发表时间：
2008-10-01
影响因子：
2.2
通讯作者：
Gruetter, Rolf
中科院分区：
文献类型：
--
作者：
Mlynarik, Vladimir;Cudalbu, Cristina;Gruetter, Rolf
在14.1T26 cm水平孔系统上获得了大鼠脑的超短回波时间质子单体素光谱。在该磁场下，成年大鼠脑组织的拟合线宽约为11赫兹。在4.2-4.4ppm的光谱范围内，首次在体内检测到了归属于磷胆碱、甘油磷胆碱和N-乙酰天冬氨酸的新的、分离的共振。此外，对乳酸、丙氨酸、γ-氨基丁酸、谷氨酸和谷胱甘肽的共振分离进行了改进。通过将体内光谱与单个代谢物的模拟光谱和测量的大分子光谱的线性组合进行拟合来估计代谢物浓度(LC模型)。计算的代谢物浓度与9.4T时的结果基本一致。这些初步结果进一步表明，将磁场强度增加到14.1T，提高了H-1核磁共振谱的光谱分辨率。这意味着啮齿类动物大脑中神经化学图谱的量化可以以更高的准确度和精密度来实现。(C)2008 Elsevier Inc.保留所有权利。
Ultra-short echo-time proton single voxel spectra of rat brain were obtained on a 14.1 T 26 cm horizontal bore system. At this field, the fitted linewidth in the brain tissue of adult rats was about 11 Hz. New, separated resonances ascribed to phosphocholine, glycerophosphocholine and N-acetylaspartate were detected for the first time in vivo in the spectral range of 4.2-4.4 ppm. Moreover, improved separation of the resonances of lactate, alanine, gamma-aminobutyrate, glutamate and glutathione was observed. Metabolite concentrations were estimated by fitting in vivo spectra to a linear combination of simulated spectra of individual metabolites and a measured spectrum of macromolecules (LCModel). The calculated concentrations of metabolites were generally in excellent agreement with those obtained at 9.4 T. These initial results further indicated that increasing magnetic field strength to 14.1 T enhanced spectral resolution in H-1 NMR spectroscopy. This implies that the quantification of the neurochemical profile in rodent brain can be achieved with improved accuracy and precision. (C) 2008 Elsevier Inc. All rights reserved.