Excitation, oscillations and wave propagation in a G-protein-based model of signal transduction in Dictyostelium discoideum.
Excitation, oscillations and wave propagation in a G-protein-based model of signal transduction in Dictyostelium discoideum.
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盘基网柄菌基于 G 蛋白的信号转导模型中的激发、振荡和波传播。
DOI：
10.1098/rstb.1995.0102
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Othmer,HG
中科院分区：
文献类型：
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作者：
Tang,Y;Othmer,HG
在早期的一篇论文（Tang & Othmer 1994）中。biosci120,25 -76)，我们开发了一种基于g蛋白的细胞黏液霉菌盘状盘基骨菌信号转导模型，并表明它可以解释Devreotes和同事（Devreoteset . 1979j）所做的灌注实验结果。Cell.80, 300 - 309;Devreotes & Steck, 1997。中国生物医学工程学报（英文版）；李志强，李志强，李志强，等。2008 .中国生物医学工程学报，32(5):557 - 561。主要的实验观测结果是cAMP的分泌量和对各种刺激的适应时间尺度，我们发现模型的预测与观测结果吻合得很好。该模型中的适应性来自于双受体介导的途径，其中一个产生刺激性G蛋白G，另一个产生抑制性G蛋白Gt。在本文中，我们使用该模型模拟Gerisch和Wick（1975）的悬浮实验。biophys。Res. comm .65, 364-370)和在培养皿中进行的细胞培养实验（Tomchik & Devreotes 1981 . science, washington .212, 443-446）。根据细胞的发育阶段和实验条件，该模型预测cAMP刺激的兴奋，持续振荡或螺旋波和目标模式。研究了不同起搏器之间的相互作用。
In an earlier paper (Tang & Othmer 1994Math. Biosci120, 25-76), we developed a G-protein-based model for signal transduction in the cellular slime mouldDictyostelium discoideumand showed that it can account for the results from perfusion experiments done by Devreotes and coworkers (Devreoteset al.1979J. Cell.80, 300-309; Devreotes & Steck 1979J. Cell Biol.80, 300-309; Dinaueret al.1980 J. Cell Biol. 86, 537—561). The primary experimental observables are the amounts of cAMP secreted and the time scale of adaptation in response to various stimuli, and we showed that the predictions of the model agree well with the observations. Adaptation in the model arises from dual receptor-mediated pathways, one of which produces a stimulatory G protein Gsand the other of which produces an inhibitory G protein Gt. In this paper we use the model to simulate the suspension experiments of Gerisch & Wick (1975Biochem. biophys. Res. Commun.65, 364—370) and the experiments done in cell cultures on Petri dishes (Tomchik & Devreotes 1981Scien, Wash.212, 443-446). The model predicts excitation to cAMP stimuli, sustained oscillations, or spiral waves and target patterns, depending on the developmental stage of the cells and experimental conditions. The interaction between different pacemakers is also studied.