Mitotic chromosome condensation requires brn1p, the yeast homologue of barren
Mitotic chromosome condensation requires brn1p, the yeast homologue of barren
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DOI：
10.1091/mbc.11.4.1293
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发表时间：
2000-04-01
影响因子：
3.3
通讯作者：
Holm, C
中科院分区：
文献类型：
--
作者：
Lavoie, BD;Tuffo, KM;Holm, C
体外研究表明，这种贫瘠的蛋白质可能作为DNA拓扑异构酶II的激活剂和/或作为非洲爪哇凝集素复合体的一个组成部分。为了更好地了解不育在体内的作用，我们在酿酒酵母中产生了不育结构基因(BRN1)的条件等位基因。我们表明，在体内，不孕不育是染色体凝聚所必需的蛋白质，它很可能作为酵母凝聚机制的内在组成部分发挥作用。与这一观点一致，我们证明了不育在染色体凝聚建立和维持的细胞周期中发挥着重要的功能。相比之下，在体内，Branen并不是DNA拓扑异构酶II的重要激活剂。最后，brn1突变体表现出额外的表型，如拉伸的染色体，异常的后期纺锤体，以及具有~gt；2C DNA含量的细胞的积累，这表明不育功能影响了染色体传递和动态的多个方面。
In vitro studies suggest that the Barren protein may function as an activator of DNA topoisomerase II and/or as a component of the Xenopus condensin complex. To better understand the role of Barren in vivo, we generated conditional alleles of the structural gene for Barren (BRN1) in Saccharomyces cerevisiae. We show that Barren is an essential protein required for chromosome condensation in vivo and that it is likely to function as an intrinsic component of the yeast condensation machinery. Consistent with this view, we show that Barren performs an essential function during a period of the cell cycle when chromosome condensation is established and maintained. Ln contrast, Barren does not serve as an essential activator of DNA topoisomerase II in vivo. Finally, brn1 mutants display additional phenotypes such as stretched chromosomes, aberrant anaphase spindles, and the accumulation of cells with >2C DNA content, suggesting that Barren function influences multiple aspects of chromosome transmission and dynamics.