Physical maps for HSV type 2 DNA with five restriction endonucleases.

Physical maps for HSV type 2 DNA with five restriction endonucleases.
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带有五种限制性内切酶的 HSV 2 型 DNA 物理图谱。

DOI:
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发表时间:
1978
影响因子:
3.8
通讯作者:
N. Wilkie
N. Wilkie
中科院分区:
医学3区
文献类型:
--
作者:
R. Cortini;N. Wilkie

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使用分子杂交技术和用进一步的限制性内切酶切割分离的限制性内切酶片段,研究了用于物理图谱的HSV-2 DNA的限制性内切酶片段的排序。已经构建了EcoRI、Hind III、Bgl II、Xba和Hpa I产生的片段的物理图谱。摩尔重量构成HSV-2基因组的各个区域中的每一个与相应的mol.重量在HSV-1基因组中。
The ordering of restriction endonuclease fragments of HSV-2 DNA for physical maps has been studied using molecular hybridization techniques and the cleavage of isolated restriction endonuclease fragments with further restriction endonucleases. Physical maps for the fragments produced by EcoRI, Hind III, Bgl II, Xba and Hpa I have been constructed. The mol. wt. of the various regions which constitute HSV-2 genome are very similar to the corresponding mol. wt. in the HSV-1 genome.