Metabolism of human embryos following cryopreservation: Implications for the safety and selection of embryos for transfer in clinical IVF
Metabolism of human embryos following cryopreservation: Implications for the safety and selection of embryos for transfer in clinical IVF
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DOI：
10.1093/humrep/del447
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发表时间：
2007-03-01
影响因子：
6.1
通讯作者：
Houghton, Franchesca D.
中科院分区：
文献类型：
--
作者：
Stokes, Paula J.;Hawkhead, Judith A.;Houghton, Franchesca D.
背景技术背景：超数胚胎的冷冻保存是人类辅助生殖中的常规操作，提供了可以解冻用于后续治疗周期的胚胎来源。然而，冷冻保存胚胎的生存能力传统上依赖于形态学评估，这是胚胎健康的不良预测因素，因为冷冻导致植入潜力的显著总体降低，并且其长期功效未知。这项研究描述了人类胚胎解冻后的代谢如何用于预测未来发育到囊胚阶段。方法：采用高效液相色谱法分析人胚胎发育第2 ~ 3天解冻后的氨基酸代谢。结果：预测冻融胚胎发育到囊胚期的准确率为87%。有发育能力的胚胎比其捕获的对应物更代谢静止。氨基酸周转也能够区分最佳I级胚胎的发育潜力P < 0.05。结论：这些数据表明，IVF中的冷冻保存是一种安全的程序，氨基酸周转可用于选择哪个冷冻保存的胚胎将发育到囊胚阶段，而无论其解冻后的等级如何。
BACKGROUND: Cryopreservation of supernumerary embryos is routinely performed in human-assisted reproduction, providing a source of embryos which can be thawed for use in subsequent treatment cycles. However, the viability of cryopreserved embryos has traditionally relied on morphological assessment, which is a poor predictor of embryo health since freezing leads to a significant overall reduction in implantation potential, and its long-term efficacy is unknown. This study describes how the post-thaw metabolism of human embryos can be used to predict future development to the blastocyst stage. METHODS: HPLC was used to analyse the post-thaw amino acid metabolism of human embryos from day 2 to day 3 of development. RESULTS: It was possible to predict with 87% accuracy which frozen-thawed embryo would develop to the blastocyst stage. Developmentally competent embryos were more metabolically quiescent than their arresting counterparts. Amino acid turnover was also capable of distinguishing between the developmental potential of the best, Grade I embryos P < 0.05. CONCLUSIONS: The data suggests that cryopreservation in IVF is a safe procedure and that amino acid turnover can be used to select which cryopreserved embryo will develop to the blastocyst stage, irrespective of their post-thaw grade.