Subtractive screen of potential limb regeneration related genes from Pachytriton brevipes
Subtractive screen of potential limb regeneration related genes from Pachytriton brevipes
复制标题
短肢厚螈潜在肢体再生相关基因的消减筛选
DOI：
10.1007/s11033-013-2946-z
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发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Huang, Xiao
中科院分区：
文献类型：
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作者：
Jiang, Dan;Zhu, Xiao-Long;Huang, Xiao
不同动物物种的再生能力差异很大。在脊椎动物中，两栖动物尤其是有尾动物，由于其受损或丢失的附肢具有很强的再生能力，已被用作研究组织再生机制的强大模型系统。短蝾螈是一种广泛分布于中国南方的蝾螈，其受损的肢体可以在几个月内完全恢复。在本研究中，我们使用改良的抑制消减杂交测定和斑点印迹筛选来鉴定参与短尾虾组织再生的候选基因。我们成功地从正向再生消减文库中分离出 81 个 EST。我们进一步通过原位杂交验证了再生胚基和非再生组织中四个候选基因Rpl11、Cirbp、Ag2和Trimx的差异表达。这些基因还通过系统发育和生物信息学分析进一步表征。总的来说，我们提供了一种比较实验方法来研究脊椎动物再生机制。
Regeneration capacity varies greatly among different animal species. In vertebrate, amphibian especially the Urodela, has been used as a powerful model system to study the mechanism of tissue regeneration because of the strong ability to regenerate their damaged or lost appendages. Pachytriton brevipes, a species of newt, which is widely distributed in south of China, can completely restore their damaged limbs within several months. In this study, we use modified suppression subtractive hybridization assay and dot-blot screening to identify candidate genes involved in tissue regeneration in P. brevipes. We successfully isolated 81 ESTs from a forward regeneration subtraction library. And we further verified the differential expression of four candidate genes, Rpl11, Cirbp, Ag2 and Trimx, between regenerating blastema and non-regeneration tissues by in situ hybridization. These genes were also be further characterized by phylogenetic and bioinformatic analysis. In general, we provided a comparative experimental approach to study the mechanisms of vertebrate regeneration.