POSTNATAL ONTOGENY OF VASOPRESSIN RECEPTORS IN THE RAT COLLECTING DUCT
POSTNATAL ONTOGENY OF VASOPRESSIN RECEPTORS IN THE RAT COLLECTING DUCT
复制标题
DOI：
10.1016/0303-7207(92)90144-u
复制
发表时间：
1992-08-01
影响因子：
4.1
通讯作者：
BUTLEN, D
中科院分区：
文献类型：
--
作者：
AMMAR, A;ROSEAU, S;BUTLEN, D
通过测定大鼠集合管中[1-（β-巯基-β，β-环戊亚甲基丙酸），2-O-甲基酪氨酸，4-苏氨酸，8-鸟氨酸，9-I-125-酪酰胺]-催产素（I-215-d（CH 2）5[Tyr（Me）2，Thr 4，Tyr-NH 29]-OVT）至分离的皮质集合管（CCD），从2- 34日龄大鼠和成年动物经胶原酶处理的肾脏显微解剖的外髓集合管（OMCD）和内髓集合管（IMCD）。CCD和OMCD受体对一系列7种加压素结构类似物的立体特异性识别结果表明，在11 ~ 16日龄大鼠和成年大鼠中识别的标记结合位点分别是同源的，并且含有V2型的主要群体和V1 a型的次要群体。在出生后的所有阶段，受体密度（表示为每平方毫米管外表面积结合10（-18）mol放射性配体）从CCD到IMCD逐渐降低。对于这三个节段，在出生后的前2周内检测到的受体数量保持恒定，20天后急剧增加，在第5周达到相应的成人水平。
The ontogenesis of vasopressin receptors in the rat collecting duct was studied by measuring the binding of [1-(beta-mercapto-beta,beta-cyclopentamethylenepropionic acid),2-O-methyltyrosine,4-threonine,8-ornithine,9-I-125-tyrosylamide]-vasotocin (I-215-d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH29]-OVT) to isolated cortical collecting ducts (CCD), outer medullary collecting ducts (OMCD) and inner medullary collecting ducts (IMCD) microdissected from collagenase-treated kidneys of 2- to 34-day-old rats and adult animals. The stereospecificity for recognition of a series of seven vasopressin structural analogues by CCD and OMCD receptors reveals that the labeled binding sites identified in 11- to 16-day-old and adult rats are homologous respectively and contain a major population of V2 type and a minor population of V1a type of vasopressin receptors. At all postnatal stages examined, the receptor density (expressed as 10(-18) mol radioligand bound per square millimeter tubular outer surface area) decreases gradually from the CCD to the IMCD. For the three segments, the numbers of receptors detected remained constant during the first 2 weeks after birth and increased sharply after 20 days to reach the corresponding adult levels during the fifth week.