High-resolution liquid chromatographic method using ultraviolet detection for determination of ondansetron in human plasma
High-resolution liquid chromatographic method using ultraviolet detection for determination of ondansetron in human plasma
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DOI：
10.1016/s0378-4347(97)00068-6
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发表时间：
1997-06-06
期刊：
影响因子：
--
通讯作者：
Caille, G
中科院分区：
文献类型：
--
作者：
Depot, M;Leroux, S;Caille, G
本文介绍了人血浆中恩丹西酮的一种简单的萃取技术和灵敏的高效液相色谱分离和定量方法。采用液-液萃取法从血浆中提取恩丹西酮，反相高效液相色谱分离，检测波长为305 nm。采用内标法进行定量。恩丹西酮的回收率为85%。在惊恐障碍研究中预测的人体血浆浓度范围内，线性良好(0.5-15 ng/ml，r(2)范围为0.9953-0.9988)。方法用于人体内恩丹西酮血药浓度的测定。
This paper describes a simple technique for extraction and a sensitive high-performance liquid chromatographic method for separation and quantitation of ondansetron in human plasma. The procedure involved liquid-liquid extraction of ondansetron from plasma, reversed-phase HPLC separation and ultraviolet detection at 305 nm. The internal standard method was applied for quantitation. The recovery of ondansetron was >85%. Linearity was good throughout the concentration range anticipated in human plasma from investigations in panic disorder (0.5-15 ng/ml, r(2) ranging from 0.9953 to 0.9988). This method was applied to the determination of plasma concentrations of ondansetron in humans.