QUANTITATIVE DETECTION OF HIV-1 DRUG-RESISTANCE MUTATIONS BY AUTOMATED DNA-SEQUENCING
QUANTITATIVE DETECTION OF HIV-1 DRUG-RESISTANCE MUTATIONS BY AUTOMATED DNA-SEQUENCING
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DOI：
10.1038/365671a0
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发表时间：
1993-10-14
期刊：
影响因子：
64.8
通讯作者：
HENFREY, RD
中科院分区：
文献类型：
--
作者：
LARDER, BA;KOHLI, A;HENFREY, RD
已经在手动和自动DNA测序程序之间进行了比较，以评估区分野生型和突变体序列的混合物的能力。这种混合的HIV-1耐药突变的定量检测最好使用荧光标记测序引物的自动化系统来实现。该方法在临床研究中有广泛的应用，包括杂合子分析。软件，自动报告混合基地的立场。
A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.