Restriction and modification in Bacillus species
Restriction and modification in Bacillus species
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DOI：
10.1007/bf00264941
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发表时间：
1977-03
期刊：
影响因子：
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通讯作者：
T. Uozumi;T. Hoshino;K. Miwa;S. Horinouchi;T. Beppu;K. Arima
中科院分区：
文献类型：
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作者：
T. Uozumi;T. Hoshino;K. Miwa;S. Horinouchi;T. Beppu;K. Arima
用噬菌体Φ 105C对63株枯草芽孢杆菌和15株其它芽孢杆菌进行了宿主特异性酶切试验，发现13株芽孢杆菌具有宿主特异性酶切。根据酶切类型将13株菌分为8个组（M、H、C、N、E、F、G、P）。M型菌株（B.枯草杆菌（Marburg 168及其衍生物和另外两个菌株）对Φ 105 C的限制作用相对较弱，对其他类群芽孢杆菌的Φ 105 C的限制比例为10 - 1：10 - 3。H、C、N、E、F、G和P群的菌株以10 - 2~10 - 8的比例限制其他群的Φ 105 C。一些菌株证实，型特异性修饰仅由最后一个宿主赋予。此外，我们还分离到一株限制性酶切缺陷型突变株。subtilismarburg 168-YS 11，其也失去了其修饰表型。
Host specific restriction was detected in 13Bacillusstrains, when 63 strains ofBacillus subtilisand 15 otherBacillusstrains were tested with phage Φ105C. These 13 strains were classified into 8 groups (M,H,C,N,E,F,G,P) by the type of restriction. M-type strains (B. subtilisMarburg 168, its derivatives, and two other strains) showed relatively weak restriction, restricting Φ105C from other groups ofBacillusby ratios of 10-1to 10-3. Strains of groups H,C,N,E,F,G, and P restricted Φ105C from other groups by ratios of 10-2to 10-8. It was confirmed with some of the strains that type-specific modification was endowed only by the last host. Furthermore, we isolated one restriction deficient mutant ofB. subtilismarburg 168-YS11, which had also lost its modification phenotype.