Histomorphometric analysis of dentinal bridge formation and pulpal inflammation.
Histomorphometric analysis of dentinal bridge formation and pulpal inflammation.
复制标题
牙本质桥形成和牙髓炎症的组织形态计量学分析。
DOI：
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发表时间：
2002
影响因子：
1.9
通讯作者：
Anthony J. Smith
中科院分区：
文献类型：
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作者：
Y. Kitasako;P. Murray;J. Tagami;Anthony J. Smith
客观化
本研究的目的是评价四种树脂复合材料作为直接盖髓材料时的牙髓反应。单独的盖髓变量的重要性和作用还没有被很好地理解；因此，组织形态计量学分析被用来分析这些变量。
方法和材料
在非人类灵长类动物的牙齿上制备了250个标准化的牙髓暴露洞。暴露的牙髓用氢氧化钙和多步自酸蚀底漆树脂复合体覆盖。术后3~60d取牙，观察牙髓反应。在灌注固定后，对组织进行脱矿、切片、染色和组织形态计量学测量。测量牙桥面积、牙髓外露直径和窝底宽度。根据定义的标准对隧道缺陷、手术残留物和牙髓炎症进行分级。
结果
与牙本质桥面积相关的变量由大到小依次为：暴露至今的时间、暴露的牙髓直径、盖髓材料和隧道缺陷。与牙髓炎症相关的变量是盖髓材料的类型和治疗。其他变量在统计上没有显著意义。
结论
树脂复合材料盖髓可提供可接受的牙髓炎和牙本质桥修复反应，与氢氧化钙相当。尽管树脂复合材料作为直接盖髓材料很有前景，但仍需进一步研究以优化其应用方案，以减少潜在的细胞毒性单体对牙髓组织的渗透。
OBJECTIVE
The purpose of this study was to evaluate pulpal responses to the use of four resin composite materials as direct pulp capping agents. The importance and effects of individual pulp capping variables are not well understood; consequently histomorphometric analysis was used to analyze these variables.
METHOD AND MATERIALS
Two hundred fifty standardized pulp-exposed cavities were prepared in nonhuman primate teeth. Exposed pulps were capped with calcium hydroxide and multistep and self-etching primer resin composites. Teeth were collected from 3 to 60 days to observe pulpal reactions. Following perfusion fixation, tissues were demineralized, sectioned, stained, and histomorphometrically measured. Bridge area, diameter of pulpal exposure, and cavity floor width were measured. Tunnel defects, operative debris, and pulpal inflammation were graded according to defined criteria.
RESULTS
The variables correlated to dentinal bridge area were, in decreasing order of significance, time elapsed since exposure, diameter of pulpal exposure, pulp capping material, and tunnel defects. The variables correlated to pulpal inflammation were the type and curing of pulp capping material. Other variables were not statistically significant.
CONCLUSION
Pulp capping with resin composite materials provided acceptable pulpal inflammatory and dentinal bridge repair responses, comparable with those of calcium hydroxide. Although resin composites are promising as direct pulp capping agents, further investigations are required to optimize their application protocols to reduce the penetration of potentially cytotoxic monomers into pulpal tissue.