Alveolar Bone Regeneration by Transplantation of Periodontal Ligament Stem Cells and Bone Marrow Stem Cells in a Canine Peri-Implant Defect Model: A Pilot Study
Alveolar Bone Regeneration by Transplantation of Periodontal Ligament Stem Cells and Bone Marrow Stem Cells in a Canine Peri-Implant Defect Model: A Pilot Study
复制标题
DOI：
10.1902/jop.2009.090249
复制
发表时间：
2009-11-01
影响因子：
4.3
通讯作者：
Lee, Yong-Moo
中科院分区：
文献类型：
--
作者：
Kim, Su-Hwan;Kim, Kyoung-Hwa;Lee, Yong-Moo
背景资料：本研究旨在探讨牙周膜干细胞（PDLSCs）和骨髓干细胞（BMSCs）在种植体周围牙槽骨再生中的作用。培养髂嵴的自体BMSCs和离体牙的PDLSCs。提取后3个月，分别将BMSC和PDLSC负载的羟基磷灰石/β-磷酸三钙（HA/TCP）（试验组）和无细胞HA/TCP（对照组）植入三个矩形鞍状种植体周围缺损中。最初准备下颌骨的左侧，8周后，也准备右侧。8周愈合期后处死动物。制备未脱钙研磨切片。通过组织形态计量学测量新骨形成和骨-种植体接触（BIC）。BMSCs和PDLSCs进行荧光标记和traced.Results：牙槽骨再生在手术创建的种植体周围鞍状缺损是更有效的测试组比对照组。BMSC组具有最高的新骨形成（在8周和16周的愈合时间分别为34.99%和40.17%），其次是PDLSC组（31.90%和36.51%）和对照组（23.13%和28.36%）。实验组在8周时新骨形成明显高于对照组，但在16周时只有BMSC组也是如此（P
Background: The present study was undertaken to evaluate the potential of periodontal ligament stem cells (PDLSCs) and bone marrow SCs (BMSCs) on alveolar bone regeneration in a canine peri-implant defect model.Methods: Four adult, male beagle dogs were used in this study. Autologous BMSCs from the iliac crests and PDLSCs from extracted teeth were cultured. Three months after extraction, BMSC- and PDLSC-loaded hydroxyapatite/beta-tricalcium phosphate (HA/TCP) (test groups) and cell-free HA/TCP (control group) were implanted in three rectangular, saddle-like peri-implant defects, respectively. The left side of the mandible was initially prepared, and after 8 weeks, the right side was also prepared. The animals were sacrificed after an 8-week healing period. Undecalcified ground sections were prepared. New bone formation and bone-to-implant contact (BIC) were measured histomorphometrically. BMSCs and PDLSCs were fluorescently labeled and traced.Results: Alveolar bone regeneration in surgically created peri-implant saddle-like defects was more effective in test groups than the control group. The BMSC group had the highest new bone formation (34.99% and 40.17% at healing times of 8 and 16 weeks, respectively) followed by the PDLSC group (31.90% and 36.51%) and control group (23.13% and 28.36%), respectively. Test groups exhibited a significantly higher new bone formation than the control group at 8 weeks, but the same was true for only the BMSC group at 16 weeks (P