Rapid screening and identification of antioxidants in the leaves of Malus hupehensis using off-line two-dimensional HPLC-UV-MS/MS coupled with a 1,1′-diphenyl-2-picrylhydrazyl assay
Rapid screening and identification of antioxidants in the leaves of Malus hupehensis using off-line two-dimensional HPLC-UV-MS/MS coupled with a 1,1′-diphenyl-2-picrylhydrazyl assay
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使用离线二维 HPLC-UV-MS/MS 结合 1,1-2-二苯基-2-苦基肼测定法快速筛选和鉴定湖北海棠叶中的抗氧化剂
DOI：
10.1002/jssc.201800007
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发表时间：
2018-06-01
影响因子：
3.1
通讯作者：
Chen, Xiaoqing
中科院分区：
文献类型：
--
作者：
Liu, Minzhuo;Huang, Xueqian;Chen, Xiaoqing
湖北海棠叶具有很强的抗氧化活性，通常作为一种健康茶食用。然而，关于其抗氧化剂的详细信息并不完整。在此，我们开发了一种有效的策略，将离线二维高效液相色谱与紫外、串联质谱学检测和1，1‘-二苯基-2-苦基肼分析相结合，快速筛选和鉴定湖北黄连叶中的抗氧化剂。在正交二维液相色谱系统中，第一维使用Venusil HILIC色谱柱，第二维安装Unusil XB-C-18色谱柱。结果初步鉴定了32种抗氧化剂，包括10个二氢查尔酮、2个黄烷酮、9个黄酮醇、4个黄酮类和7个酚酸类化合物，其中23个化合物为首次从湖北黄连叶中分离和鉴定。据我们所知，这是第一次系统地研究湖北微囊藻叶片中的抗氧化剂。结果表明，该方法是一种快速研究抗氧化剂的有效方法，特别是对复杂体系中的空泡化合物和次要化合物。
The leaves of Malus hupehensis have a strong antioxidant activity and are commonly consumed as a healthy tea. However, detailed information about its antioxidants is incomplete. Herein, we developed an effective strategy based on combining offline two-dimensional high-performance liquid chromatography with ultraviolet and tandem mass spectrometry detection with a 1,1'-diphenyl-2-picrylhydrazyl assay to rapidly screen and identify the antioxidants from the leaves of M. hupehensis. In the orthogonal two-dimensional liquid chromatography system, a Venusil HILIC column was used for the first dimension, while a Universil XB-C-18 column was installed in the second dimension. As a result, 32 antioxidants, including ten dihydrochalcones, two flavanones, nine flavonols, four flavones, and seven phenolic acids were tentatively identified, out of which 23 compounds, as far as we know, were isolated and characterized from the leaves of M. hupehensis for the first time. To the best of our knowledge, this is the first systematic investigation of the antioxidants from the leaves of M. hupehensis. The results indicated that the proposed method is an efficient technique to rapidly investigate antioxidants, especially for coeluted and minor compounds in a complex system.