AUREOCOCCUS ANOPHAGEFFERENS (PELAGOPHYCEAE) GENOMES IMPROVE EVALUATION OF NUTRIENT ACQUISITION STRATEGIES INVOLVED IN BROWN TIDE DYNAMICS
AUREOCOCCUS ANOPHAGEFFERENS (PELAGOPHYCEAE) GENOMES IMPROVE EVALUATION OF NUTRIENT ACQUISITION STRATEGIES INVOLVED IN BROWN TIDE DYNAMICS
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DOI：
10.1111/jpy.13221
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发表时间：
2021-12-14
影响因子：
2.9
通讯作者：
Wilhelm, Steven W.
中科院分区：
文献类型：
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作者：
Gann, Eric R.;Truchon, Alexander R.;Wilhelm, Steven W.
海洋植物Aureococcus anophagefferens在三大洲的海湾造成有害的棕色潮花。Aureococcus anophagefferens是第一个进行基因组测序的有害藻华物种，这一进展证明了基因对于适应褐潮期间盛行的环境条件至关重要。为了扩展该物种可用的基因组工具，组装了四种菌株的基因组，包括三种新测序的菌株和一种从公开数据组装的菌株。这些基因组的大小为57.11 ~ 73.62 Mb，编码13，191 ~ 17，404种潜在蛋白质。所有菌株共享相似的90%，其编码的蛋白质确定的同源性搜索，并共享大多数功能的直系同源物确定的KEGG，虽然每个菌株也具有编码序列具有独特的功能。与原始参考基因组一样，本研究中组装的基因组具有假设在水华增殖中重要的基因，包括参与有机化合物代谢和低光下生长的基因。跨菌株信息学和培养实验表明，嘌呤的利用是褐潮有机氮的一个潜在的重要来源。对来自褐潮事件的元转录组的分析表明，与从所有可用基因组生成的数据库（类似于43%）相比，使用单个基因组产生较低的读取映射百分比（类似于30%的文库读取），这表明可以从扩展基因组空间获得关于水华生态的新信息。这项工作表明，继续需要测序生态相关的藻类，以了解基因组的潜力和它们在环境中的生态。
The pelagophyte Aureococcus anophagefferens causes harmful brown tide blooms in marine embayments on three continents. Aureococcus anophagefferens was the first harmful algal bloom species to have its genome sequenced, an advance that evidenced genes important for adaptation to environmental conditions that prevail during brown tides. To expand the genomic tools available for this species, genomes for four strains were assembled, including three newly sequenced strains and one assembled from publicly available data. These genomes ranged from 57.11 to 73.62 Mb, encoding 13,191-17,404 potential proteins. All strains shared similar to 90% of their encoded proteins as determined by homology searches and shared most functional orthologs as determined by KEGG, although each strain also possessed coding sequences with unique functions. Like the original reference genome, the genomes assembled in this study possessed genes hypothesized to be important in bloom proliferation, including genes involved in organic compound metabolism and growth at low light. Cross-strain informatics and culture experiments suggest that the utilization of purines is a potentially important source of organic nitrogen for brown tides. Analyses of metatranscriptomes from a brown tide event demonstrated that use of a single genome yielded a lower read mapping percentage (similar to 30% of library reads) as compared to a database generated from all available genomes (similar to 43%), suggesting novel information about bloom ecology can be gained from expanding genomic space. This work demonstrates the continued need to sequence ecologically relevant algae to understand the genomic potential and their ecology in the environment.