Mixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17: evidence for high frequency reassortment in the vector.
Mixed infection of Culicoides variipennis with bluetongue virus serotypes 10 and 17: evidence for high frequency reassortment in the vector.
复制标题
库蠓与蓝舌病毒血清型 10 和 17 的混合感染：载体中高频重配的证据。
DOI：
10.1099/0022-1317-68-9-2319
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Ramig,RF
中科院分区：
文献类型：
--
作者：
Samal,SK;el-Hussein,A;Holbrook,FR;Beaty,BJ;Ramig,RF
美国蓝舌病病毒 (BTV) 的主要媒介物种是库蠓 (Culicoides variipennis)，经口感染 BTV 血清型 10、BTV 血清型 17 或两种病毒的混合物。经过一段时间的外部孵化后，受感染果蝇的病毒回收率很低。对单次感染果蝇的子代病毒进行电泳分析表明，只能从这些果蝇中分离出亲本电泳型。相比之下，对混合感染果蝇的病毒进行电泳分析表明，11 只病毒阳性果蝇中的 8 只产生了具有重配电泳型的病毒后代。重配后代的比例从 7% 到 78%（平均 42%）不等，具体取决于果蝇个体。对重配后代病毒中基因组片段亲本起源的分离分析表明，虽然大多数片段的重配是随机的，但可能已经发生了从 17 型亲本中选择基因组片段 8 的情况。对单个混合感染果蝇的分离分析表明，每只果蝇产生了一组相对独特的重配体，但特定的电泳型从单个果蝇中重复分离出来。这些数据表明载体物种 C.variipennis 是 BTV 基因组片段高频重配的允许宿主。
The primary vector species for bluetongue virus (BTV) in the United States,Culicoides variipennis,was orally infected with BTV serotype 10, BTV serotype 17, or a mixture of the two viruses. The recovery of virus from the infected flies was low following a period of extrinsic incubation. Electrophoretic analysis of progeny virus from singly infected flies revealed that only the parental electropherotype could be isolated from those flies. In contrast, electrophoretic analysis of virus from mixedly infected flies revealed that eight of the 11 virus-positive flies produced virus progeny with reassortant electropherotypes. The proportion of reassortant progeny varied from 7 to 78% (mean 42%), depending on the individual fly. Analysis of segregation of the parental origin of genome segments in the reassortant progeny virus suggested that, while reassortment of most segments was random, selection for genome segment 8 from the type 17 parent may have occurred. Analysis of segregation in individual mixedly infected flies showed that each fly yielded a relatively unique set of reassortants, but that specific electropherotypes were isolated repeatedly from individual flies. These data indicated that the vector species C.variipenniswas a permissive host for high frequency reassortment of genome segments of BTV.