Molecular identification of a recent type of canine distemper virus in Japan by restriction fragment length polymorphism.
Molecular identification of a recent type of canine distemper virus in Japan by restriction fragment length polymorphism.
复制标题
利用限制性片段长度多态性对日本新型犬瘟热病毒进行分子鉴定。
DOI：
10.1292/jvms.60.1209
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
C. Kai
中科院分区：
文献类型：
--
作者：
K. Ohashi;K. Iwatsuki;K. Nakamura;T. Mikami;C. Kai
应用限制性片段长度多态性分析技术对犬瘟热病毒（CDU）疫苗株和新野毒进行了鉴别。通过逆转录-聚合酶链反应扩增部分血凝素基因的病毒基因组。用EcoRV酶切后，将新分离株的PCR产物切割成两个片段，这两个片段不同于包括所有疫苗株在内的旧株的未切割形式。该方法适用于新鲜或储存的感染犬脑、脾和外周血单个核细胞。这种分子方法对于确定疫苗接种后CDV感染的病原体是有用的。
Restriction fragment length polymorphism analysis was used to differentiate recent field viruses of canine distemper virus (CDU) from vaccine strains. Virus genomes were amplified by using reverse transcriptase-polymerase chain reaction in part of the haemagglutinin gene. After digestion with EcoRV, the PCR products of recent field isolates were cut into two fragments that differ from the uncut form of old strains including all of vaccine strains. This method could be applied to fresh or stored brains, spleens and peripheral blood mononuclear cells of infected dogs. This molecular approach is useful for determining the causative agent of postvaccinated CDV infection.