Liquid Crystal ordering of DNA Dickerson Dodecamer duplexes with different 5’- Phosphate terminations
Liquid Crystal ordering of DNA Dickerson Dodecamer duplexes with different 5’- Phosphate terminations
复制标题
具有不同 5- 磷酸盐末端的 DNA Dickerson Dodecamer 双链体的液晶排序
DOI：
10.1080/15421406.2019.1581706
复制
发表时间：
2019
影响因子：
0.7
通讯作者：
T. P. Fraccia
中科院分区：
文献类型：
--
作者：
M. Todisco;Gregory P Smith;T. P. Fraccia
摘要DNA寡聚体在浓水溶液中液晶相的出现主要取决于DNA双链体之间的端对端相互作用，这种相互作用可以通过末端碱基对的芳香堆积或互补悬挂末端的配对来提供。在这里，我们研究了三个平端12个碱基长的DNA双链合成的羟基，磷酸和三磷酸5 '端的LC行为。我们的实验特征的浓度-温度相图，我们定量估计的端到端的堆积自由能，通过比较经验数据与粗粒度的线性聚集模型的预测。LC排序的保存，即使在存在的庞大的和高度带电的三磷酸基团，表明有吸引力的堆积相互作用仍然存在，并能够诱导线性聚集的DNA双链体。这一发现加强了发色体样自组装对于线性聚合核酸的益生元形成的潜在作用。
Abstract The onset of liquid crystal (LC) phases in concentrated aqueous solutions of DNA oligomers crucially depends on the end-to-end interaction between the DNA duplexes, which can be provided by the aromatic stacking of the terminal base-pairs or by the pairing of complementary dangling-ends. Here we investigated the LC behavior of three blunt-end 12-base-long DNA duplexes synthesized with hydroxyl, phosphate and triphosphate 5’-termini. We experimentally characterized the concentration-temperature phase diagrams and we quantitatively estimated the end-to-end stacking free energy, by comparing the empirical data with the predictions of coarse-grained linear aggregation models. The preservation of LC ordering, even in presence of the bulky and highly charged triphosphate group, indicates that attractive stacking interactions are still present and capable of induce linear aggregation of the DNA duplexes. This finding strengthens the potential role of chromonic like self-assembly for the prebiotic formation of linear polymeric nucleic acids.
影响因子：
5.5
作者：
Drsata, Tomas;Perez, Alberto;Orozco, Modesto;Morozov, Alexandre V.;Sponer, Jiri;Lankas, Filip
通讯作者：
Lankas, Filip
影响因子：
2.9
作者：
Lu, YJ;Weers, B;Stellwagen, NC
通讯作者：
Stellwagen, NC
影响因子：
56.9
作者：
Kilchherr, Fabian;Wachauf, Christian;Dietz, Hendrik
通讯作者：
Dietz, Hendrik