Surprising production of a new urdamycin derivative by S-fradiae ΔurdQ/R
Surprising production of a new urdamycin derivative by S-fradiae ΔurdQ/R
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DOI：
10.1016/j.jbiotec.2007.02.018
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发表时间：
2007-05-31
影响因子：
4.1
通讯作者：
Bechthold, A.
中科院分区：
文献类型：
--
作者：
Fedoryshyn, M.;Nur-e-Alam, M.;Bechthold, A.
一株（S.在分别编码dTDP-己糖-3，4-脱氢酶和dTDP-己糖-4-酮还原酶的urdQ和urdR中具有突变的frauricase Δ urdQ/R）产生了具有聚酮化合物结构变化的新的urdamycin类似物（urdamycin X）。用核磁共振波谱法鉴定了乌达霉素X的结构。在两种S. fragrant Delta urdQ，在S. fragrance Delta urdR或S. fragrance A0，一个缺乏所有糖基转移酶基因的突变体。S.果糖Delta urdQIR恢复了urdamycin A的生产，表明突变没有引起任何极性效应。(C)2007 Elsevier B. V.保留所有权利。
A strain (S. fradiae Delta urdQ/R) with mutations in urdQ and urdR encoding a dTDP-hexose-3,4-dehydratase and a dTDP-hexose-4-ketoreductase, respectively, produces a new urdamycin analogue (urdamycin X) with changes in the polyketide structure. The structure of urdamycin X has been elucidated by NMR spectroscopy. Urdamycin X was not detectable, even in small amounts, in either S. fradiae Delta urdQ, in S. fradiae Delta urdR or in S. fradiae A0, a mutant lacking all glycosyltransferase genes. Complementation of S. fradiae Delta urdQIR restored urdamycin A production indicating that the mutations did not cause any polar effect. (C) 2007 Elsevier B.V. All rights reserved.