Peptide Array-Based Characterization and Design of ZnO-High Affinity Peptides
Peptide Array-Based Characterization and Design of ZnO-High Affinity Peptides
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DOI：
10.1002/bit.22772
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发表时间：
2010-08-15
影响因子：
3.8
通讯作者：
Honda, Hiroyuki
中科院分区：
文献类型：
--
作者：
Okochi, Mina;Sugita, Tomoya;Honda, Hiroyuki
具有对ZnO的亲和力和产生ZnO纳米颗粒的能力的肽已经引起了人们对在环境条件下具有组成均匀性的纳米级构建块的自组装和模板化的关注。在这项研究中，我们分析了ZnO结合肽，EAHVMHK-VAPRP，这是确定使用噬菌体展示肽库的特异性结合位点。对ZnO纳米粒子的肽结合测定进行了使用纤维素膜上使用斑点法合成的肽。使用随机旋转的氨基酸的ZnO结合肽，125点合成的肽进行了测定。对ZnO纳米粒子的肽结合活性变化很大。这表明ZnO结合不依赖于这些肽的总疏水性或其他物理参数，而是ZnO识别这些肽的特定氨基酸排列。此外，发现几种肽与原始肽相比显示出更高的结合能力。通过从两端缩短的逐步序列研究了EAHVMHKVAPRP肽中重要结合位点的鉴定。有趣的是，发现两个ZnO结合位点为6聚体肽：HVMHKV和HKVAPR。通过氨基酸取代HKVAPR鉴定的肽被发现对ZnO纳米颗粒显示出高亲和力和特异性。Biotechnol. Bioeng. 2010;106：845-851。(C)2010 Wiley Periodicals，Inc.
Peptides with both an affinity for ZnO and the ability to generate ZnO nanoparticles have attracted attention for the self-assembly and templating of nanoscale building blocks under ambient conditions with compositional uniformity. In this study, we have analyzed the specific binding sites of the ZnO-binding peptide, EAHVMHK-VAPRP, which was identified using a phage display peptide library. The peptide binding assay against ZnO nanoparticles was performed using peptides synthesized on a cellulose membrane using the spot method. Using randomized rotation of amino acids in the ZnO-binding peptide, 125 spot-synthesized peptides were assayed. The peptide binding activity against ZnO nanoparticles varied greatly. This indicates that ZnO binding does not depend on total hydrophobicity or other physical parameters of these peptides, but rather that ZnO recognizes the specific amino acid alignment of these peptides. In addition, several peptides were found to show higher binding ability compared with that of the original peptides. Identification of important binding sites in the EAHVMHKVAPRP peptide was investigated by shortened, stepwise sequence from both termini. Interestingly, two ZnO-binding sites were found as 6-mer peptides: HVMHKV and HKVAPR. The peptides identified by amino acid substitution of HKVAPR were found to show high affinity and specificity for ZnO nanoparticles. Biotechnol. Bioeng. 2010;106: 845-851. (C) 2010 Wiley Periodicals, Inc.