The effect of decoy molecules on the activity of the P450BM3 holoenzyme and a heme domain peroxygenase variant
The effect of decoy molecules on the activity of the P450BM3 holoenzyme and a heme domain peroxygenase variant
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诱饵分子对 P450BM3 全酶和血红素结构域过氧化酶变体活性的影响
DOI：
10.1016/j.catcom.2019.03.004
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发表时间：
2019
影响因子：
3.7
通讯作者：
S. G.
中科院分区：
文献类型：
--
作者：
Dezvarei,S.; Shoji;O.; Watanabe;Y.; Bell;S. G.
基于脂肪酸和氨基酸的组合的全氟诱饵分子用于增强P450 Bm 3的羟基化、环氧化和磺基氧化反应。氨基酸衍生的第二代诱饵分子与速率加速变体R19（R47 L/Y51 F/H171 L/Q307 H/N319 Y）的组合显示出最高的氧化速率。Thr 268突变为Glu（Bm 3 TE）使血红素结构域转化为利用过氧合酶的H2 O2。该Bm 3 TE变体对所有测试的底物显示出显著的过氧合酶活性，优选甲硫基苯磺基氧化。然而，诱饵分子的添加并没有提高该变体的效率。
Perfluorinated decoy molecules based on a combination of fatty and amino acids were used to enhance hydroxylation, epoxidation and sulfoxidation reactions of P450Bm3. The combination of amino acid derived second generation decoy molecules, with the rate accelerating variant R19 (R47L/Y51F/H171L/Q307H/N319Y) displayed the highest oxidation rates. Mutation of Thr268 to Glu (Bm3TE) converted the heme domain to a H2O2utilising peroxygenase. This Bm3TE variant displayed significant peroxygenase activity towards all the substrates tested with a preference for methylthiobenzene sulfoxidation. However, the addition of decoy molecules did not improve the efficiency of this variant.