Isolation and characterization of naive follicular dendritic cells
Isolation and characterization of naive follicular dendritic cells
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幼稚滤泡树突状细胞的分离和表征
DOI：
10.1016/j.molimm.2011.11.010
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发表时间：
2012
影响因子：
3.6
通讯作者：
Kenta Usui
中科院分区：
文献类型：
--
作者：
DOHI;Toshifumi;仲野徹;Kenta Usui
滤泡树突状细胞（FDC）是淋巴组织滤泡内的一种特异性抗原提呈细胞，可提呈同源B细胞。FDC还支持B细胞的存活和增殖，从而导致生发中心的形成。因此，FDC在体液免疫应答中发挥核心作用。然而，FDC的分子和功能特征在很大程度上是未知的，因为由于FDC在淋巴组织中的数量非常少以及在体外机械和化学应力下的脆弱性，很难分离和分析FDC。本研究建立了一种新的流式细胞术分离纯化FDC的方法，并对其表型和功能特性进行了分析。分离的FDC占未处理小鼠脾细胞的约0.2%，为CD 45 −、FDC-M2+和ICAM-1+，支持B细胞的存活和LPS诱导的增殖。我们还发现，针对B细胞活化因子TNF家族（BAFF）的中和抗体在LPS存在下抑制FDC依赖性B细胞增殖，但不抑制存活，证明FDC衍生的BAFF参与B细胞增殖的证据。
Follicular dendritic cells (FDC) are specialized antigen-presenting cells to cognate B cells in the follicle of the lymphoid tissues. FDC also support survival and proliferation of the B cells, leading to the germinal center formation. FDC therefore play a central role in humoral immune responses. However, molecular and functional characteristics of FDC are largely unknown, because it is difficult to isolate and analyze FDC due to a very small number of FDC in the lymphoid tissues and the fragility by mechanical and chemical stresses in vitro. In this report, we established a novel method for FDC isolation from the spleen of naïve mice by flow cytometry and analyzed the phenotypical and functional characteristics. The isolated FDC, which accounted for ∼0.2% of the spleen cells of naïve mice, were CD45−, FDC-M2+, and ICAM-1+, and supported the survival and LPS-induced proliferation of B cells. We also showed that a neutralizing antibody against B cell activating factor TNF family (BAFF) suppressed FDC-dependent B cell proliferation in the presence of LPS, but not survival, demonstrating the evidence that FDC-derived BAFF is involved in B cell proliferation.